Technical Data
Osteocalcin, aa4-9 (Bone-Gla-Protein, BGP)
Osteocalcin, also known as Bone-Gla-Protein (BGP), is a single chain (Mr=5800) vitamin K dependent protein produced by osteoblasts. It is found in high concentrations in bone. Post-translational modification by a vitamin K dependent carboxylase produces three g-carboxyglutamic acid residues at positions 17, 21 and 24. The mature protein contains 49 amino acids and a single intrachain disulfide bond joining Cys23 to Cys29. The secondary structure is highly calcium dependent and contains 14% alpha-helix, 20% beta-sheet and 67% random form in the presence of calcium, and 1% alpha-helix, 20% beta-sheet and 79% random form in the absence of calcium. BGP binds to phospholipid vesicles in the presence of calcium ions (Kd=6 x 10e-6 M). BGP also binds hydroxyapatite and is an efficient inhibitor of hydroxyapatite seeded crystal growth, suggesting a regulatory role in bone mineralization. Although the fundamental role of BGP in vivo remains elusive, in vitro experimentation suggests roles in both bone resorption and bone formation. BGP has been shown to function as a chemo-attractant in an in vitro macrophage mediated bone resorption system. The serum concentration of BGP has been linked to bone formation rates, and BGP levels are currently used in research assessment of osteoporosis and other metabolic bone disease states.

Suitable for use in Immunohistochemistry and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: Frozen sections
Immunohistochemistry (FFPE): 10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG16D627Affinity Purified
100ug-20CBlue IceBovineMouse
~2mg/ml (after reconstitution)
Bovine osteocalcin (P02820).
Purified by affinity chromatography
Supplied as a lyophilized powder from PBS, pH 7.4, 1% BSA. Reconstitute with 50ul sterile ddH20. Dilute further with PBS, pH 7.4, 1% BSA, 0.1% sodium azide.
Recognizes aa4-9 of bovine osteocalcin. Species Crossreactivity: human, goat, sheep, canine, porcine, rabbit, chicken. Does not crossreact with mouse or rat osteocalcin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Price, P.A., et al., PNAS 73: 1447 (1976). 2. Price, P.A., Vitam. Horm. 42: 65 (1985). 3. Koyama, N., et al., J. Immunol. Methods 139: 17 (1991). 4. Vergnaud, P., et al., J. Clin. Endocrinol. Metab. 82: 719 (1997).