Technical Data
O8060-17A
Osteocalcin (Bone-Gla-Protein, BGLAP, BGP, Bone gamma Carboxyglutamate Protein, Gamma Carboxyglutamic Acid-containing Protein, PMF1)
Description:
Osteocalcin belongs to the osteocalcin/matrix Gla protein family and constitutes 1-2% of the total bone protein. It is a 49aa single chain vitamin K dependent protein, made by osteoblasts, and is a major component of the noncollagenous bone matrix. Post translational modification by a vitamin K dependent carboxylase produces three g carboxyglutamic acid residues at positions 17, 21 and 24, giving it a high affinity for calcium. It also binds strongly to apatite.

Applications:
Suitable for use in sandwich ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1-10ug/ml under reducing or non-reducing conditions.
Immunohistochemistry (Frozen, paraffin): 1-10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a9A87Affinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceBovineMouse
Concentration:
~1mg/ml
Immunogen:
Full length bovine Osteocalcin. Cellular Localization: Secreted Epitope: Includes the gamma-carboxylated residue at position 17.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4, 1% BSA, 0.1% sodium azide.
Specificity:
Recognizes bovine Osteocalcin. Species Crossreactivity: human, rat, canine, chicken, goat, porcine, rabbit, sheep. Does not crossreact with mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.