		Technical Data
O8060-17A	Osteocalcin (Bone-Gla-Protein, BGLAP, BGP, Bone gamma Carboxyglutamate Protein, Gamma Carboxyglutamic Acid-containing Protein, PMF1)

Description:
Osteocalcin belongs to the osteocalcin/matrix Gla protein family and constitutes 1-2% of the total bone protein. It is a 49aa single chain vitamin K dependent protein, made by osteoblasts, and is a major component of the noncollagenous bone matrix. Post translational modification by a vitamin K dependent carboxylase produces three g carboxyglutamic acid residues at positions 17, 21 and 24, giving it a high affinity for calcium. It also binds strongly to apatite.

Applications:
Suitable for use in sandwich ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1-10ug/ml under reducing or non-reducing conditions.
Immunohistochemistry (Frozen, paraffin): 1-10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG2a	9A87	Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Bovine	Mouse

Concentration:

~1mg/ml

Immunogen:

Full length bovine Osteocalcin. Cellular Localization: SecretedEpitope: Includes the gamma-carboxylated residue at position 17.

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in 10mM PBS, pH 7.4, 1% BSA, 0.1% sodium azide.

Specificity:

Recognizes bovine Osteocalcin. Species Crossreactivity: human, rat, canine, chicken, goat, porcine, rabbit, sheep. Does not crossreact with mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.