Technical Data
O8063-12
Osteonectin (SPARC, Secreted Protein, Acidic, Cysteine-rich, ON, OSN)
Description:
SPARC, also known as Osteonectin and basement membrane protein BM-40, is a phosphorylated glycoprotein with calcium-binding domains. It is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. SPARC may be involved in cell proliferation, repair of tissue damage and morphogenetic processes such as modeling of extracellular matrix.

Applications:
Suitable for use in ELISA, Flow Cytometry and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1-0.2ug/ml
Flow Cytometry: 3-10ug/ml per 10e6 cells.
ELISA: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Reconstitute with sterile PBS. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceMouseGoat
Concentration:
~0.2mg/ml (after reconstitution)
Immunogen:
Recombinant corresponding to Ala18-Ile302 from mouse Osteonectin expressed in NSO cells (P07214). Species Sequence Homology: rat; 97%, human, canine, bovine; 92%, chicken; 83%
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity:
Recognizes mouse Osteonectin. Shows <1% crossreactivity with recombinant human Osteonectin in direct ELISAs and Western Blots.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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