Technical Data
p47 phox (NCF1, NOXO2, Neutrophil cytosolic factor 1, 47kD Chronic granulomatous disease, autosomal 1)
NADPH Oxidase (nicotinamide adenine dinucleotide phosphate-oxidase) complex is composed of p47 phox (cytosolic component bound to PKC that localizes to p22), p67 phox (cytosolic component that interacts with activated Rac to dock on the glycoprotein p91 subunit (gp91) of membrane bound cytochrome b), Rac2 (Rho family of GTPases), b558 (a cytochrome b transmembrane protein dimer made up of gp91 phox/p22 phox). The activated complex is responsible for the respiratory burst in neutrophils by the multiple phosphorylations of p47 phox by PKC. Neutrophil cytosolic factor 1 (NCF1) is a 47kD cytosolic subunit of the multi-protein complex known as NADPH oxidase. Expression of NCF1 is restricted to granulocytes, monocytes and B lymphocytes. Defects in NCF1 have been reported to be a cause of autosomal recessive chronic granulomatous disease.

Suitable for use in Western Blot and Electron microscopy. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000. Detects p47-phox in RIPA lysates from HL-60 cells.
Optimal dilutions to be determined by the researcher.

Recommended Control:
HL-60 cell lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20CBlue IceHumanRabbit
Not determined
GST fusion protein corresponding to full length human p47-phox
Supplied as a liquid in buffer, 0.05% sodium azide, 30% glycerol.
Recognizes human p47-phox, Mr ~47kD. Species Crossreactivity: rat. Reported crossreactivity with bovine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Wientjes, F.B., et al., J. Leukocyte Biol. 61: 303-312, 1997. 2. Rodaway, A.R., et al., Mol. Cell. Biol. 10: 5388-5396, 1990. 3. Review article: Babior, B.M., Blood 93: 14-64-1476, 1999.