		Technical Data
P1001-24H	p47 phox (NCF1, NOXO2, p47phox, neutrophil cytosolic factor 1, 47kD, chronic granulomatous disease, autosomal 1, Neutrophil cytosolic factor-1 (47kD)

Description:
Neutrophil cytosolic factor 1 (NCF1) is a 47kD cytosolic subunit of the multi-protein complex known as NADPH oxidase. Expression of NCF1 is restricted to granulocytes, monocytes and B lymphocytes. Defects in NCF1 have been reported to be a cause of autosomal recessive chronic granulomatous disease.

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:250-1:1000 detected p47-phox in RIPA lysates from HL-60 cells.
Immunoprecipitation: 10ul immunoprecipitates p47-phox from 500ug of HL-60 RIPA lysate.
Immunocytochemistry: 1:250-1:2000 dilution showed positive cytosolic immunostaining for p47-phox in HL-60 cells fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Serum

Size		Storage	Shipping	Source	Host
200ul		4°C (-20°C Glycerol)	Blue Ice	Human	Rabbit

Concentration:

Not determined

Immunogen:

GST fusion protein corresponding to full-length human p47-phox

Purity:

Serum

Form

Supplied as a liquid in 5mM EDTA, 0.02% sodium azide. before the addition of glycerol to 40%.

Specificity:

Recognizes human p47-phox, Mr 47kD. Species Crossreactivity: Reported crossreactivity with bovine.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Javesghani, D., et al., Am. J. Respir. Crit. Care Med. 165: 412-418 (2002). 2. Pagano, P.J., et al., Proc. Natl. Acad. Sci. USA 94: 14,483-14,488 (1997).