Technical Data
p47 phox (NCF1, NOXO2, p47phox, neutrophil cytosolic factor 1, 47kD, chronic granulomatous disease, autosomal 1, Neutrophil cytosolic factor-1 (47kD)
Neutrophil cytosolic factor 1 (NCF1) is a 47kD cytosolic subunit of the multi-protein complex known as NADPH oxidase. Expression of NCF1 is restricted to granulocytes, monocytes and B lymphocytes. Defects in NCF1 have been reported to be a cause of autosomal recessive chronic granulomatous disease.

Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:250-1:1000 detected p47-phox in RIPA lysates from HL-60 cells.
Immunoprecipitation: 10ul immunoprecipitates p47-phox from 500ug of HL-60 RIPA lysate.
Immunocytochemistry: 1:250-1:2000 dilution showed positive cytosolic immunostaining for p47-phox in HL-60 cells fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
200ul4C (-20C Glycerol)Blue IceHumanRabbit
Not determined
GST fusion protein corresponding to full-length human p47-phox
Supplied as a liquid in 5mM EDTA, 0.02% sodium azide. before the addition of glycerol to 40%.
Recognizes human p47-phox, Mr 47kD. Species Crossreactivity: Reported crossreactivity with bovine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Javesghani, D., et al., Am. J. Respir. Crit. Care Med. 165: 412-418 (2002). 2. Pagano, P.J., et al., Proc. Natl. Acad. Sci. USA 94: 14,483-14,488 (1997).