Technical Data
P1001-24P
p67 phox (NCF2, Neutrophil Cytosol Factor 2, 67kD Neutrophil Oxidase Factor, NCF-2, Neutrophil NADPH Oxidase Factor 2, NOXA2)
Description:
Activation of NADPH-oxidase dependent superoxide generation requires the participation of several cytosolic factors including p47phox, p67phox, p40phox and Rac (1 or 2). In the resting cell, p47phox, p67phox, and p40phox form a large molecular weight complex which can be recovered from the cytosolic fraction. Activation of the NADPH oxidase is initiated by the assembly of p47phox, p67phox, and Rac with cytochrome b558 in a 1:1:1:1 complex in the plasma membrane. p47phox, p67phox, and Rac exhibit cooperative binding, since increasing the concentration of any one component lowers the EC50 of the other components. A great deal of current research involves understanding the protein protein interactions, and how these change with the activation state.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-2.0ug/ml detects p67-phox in RIPA lysates from RAW 264.7 cells.
Optimal dilutions to be determined by researcher.

Recommended Control:
Rat brain tissue, RAW264.7 cell lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptides corresponding to the first 15aa from the N-terminal and the last 15aa on the C-terminal of mouse p67-phox (KLH).
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes mouse p67-phox at ~67kD. Species Crossreactivity: rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Mizuki, K., et al., Eur. J. Biochem. 251: 573-582 (1998). 2. Okamura, N., et al., J. Clin. Invest. 85: 15831587 (1990). 3. Ellson, C., et al., EMBO J. 25: 4468-4478 (2006). 4. Vignais, P.V., et al., Cell. Mol. Life Sci. 59: 1428-1459 (2002).