Technical Data
P1005-07
p300 (EP300, E1A binding protein p300, E1A-associated protein p300, Histone acetyltransferase p300, KAT3B, P300, p300 HAT)
Description:
p300 is a nuclear phosphoprotein which was originally shown to specifically bind to adenovirus E1A protein. The binding of p300 to E1A occurs in the conserved region 1 (CR1) of E1A at a site which is close to but non-overlapping with that for the Rb family of proteins including p105 and p107. p300 has been shown to bind to the extreme N-terminal region requiring the presence of positively charged residues at positions 2 and 3 of E1A via a zinc-finger motif in the C-terminal portion of p300. While the binding of p300 and Rb to E1A is independent, it has been shown that upon binding, E1A mediates the physical contact between the two proteins. The p300 protein is actively phosphorylated in both quiescent and proliferating cells however the amount of phosphorylation increases during progression from late G1 into M phase of the cell cycle. In addition to the demonstrated binding of p300 to E1A it has also been shown to bind to other molecules including DNA (in a site specific manner) and to components of the TATA binding protein complexes (TBP). The TBP complex association is most likely with TFUD and occurs in a protein-protein dependent rather than DNA dependent manner and, in such a complex, p300 functions as a co-activator of transcription. CPB is similar both structurally and functionally to p300.
Applications:
Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Immunofluorescence: 1-5ug/ml
Immunohistochemistry (Paraffin): 1-5ug/ml; Staining of formalin fixed, paraffin embedded tissue requires HIER pretreatment
Immunoprecipitation: 1ug/sample; Autoradiographic detection.
Western Blot: 1ug/ml; Chemiluminescence detection.
Optimal dilutions to be determined by the researcher.
Positive Control:
A431, HeLa or ML-1 cells
Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG13G230Affinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Recombinant human p300 protein.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes human p300. Does not react with mouse or rat p300.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Yee, S. and Branton, P., Virology 147: 142-153 (1985) 2. Egan, C., et al., Mol. Cell Biol. 8: 3995-3959 (1988). 3. Yaciuk, P. and Moran, W., Mol. Cell Biol. 11: 5389:5397 (1991). 4. Shisler, L., et al., J. Virol. 70: 68-77 (1996). 5. Wang, H.G., et al., J. Virol. 69: 79177924 (1995). 6. Wang, H.G., et al., J. Virol. 67: 476-488 (1993). 7. Howe, J.A. and Bayley, S.T., Virology 186: 15-24 (1992). 8. Eckner, R., et al., Genes Dev. 8: 869-884 (1994). 9. Banerjee, A.C., et al., Oncogene 9: 1733-1737 (1994). 10. Rikitake, Y. and Moran, E., Mol. Cell. Biol. 11: 2826-2836 (1992). 11. Abraham, S.E., et al., Oncogene 8: 1639-1647 (1993).