Technical Data
P1775-03
PACAP-38 (Pituitary Adenylate Cyclase Activating Polypeptide)
Description:
Pituitary adenylate cyclase activating peptide (PACAP), originally isolated from ovine hypothalamus, belongs to the VIP-family of peptides. It occurs in two biologically active forms, PACAP-38 and PACAP-27. PACAP is abundant in the brain, but can be also found in the respiratory and gastrointestinal tracts. Absorption with 10-100ug immunogen per ml diluted antiserum abolishes the staining, while PRP, VIP, PHI, CRF, oxytocin and vasopressin do not.

Applications:
Suitable for use in Immunofluorescence Microscopy and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunofluorescence Microscopy (Frozen sections): 1:400-1:800 with overnight incubation at 4C.
Optimal dilutions to be determined by the researcher.

Positive Control:
Stefanini-fixed frozen sections of rat duodenum or hypothalamus.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile 40-50% glycerol, ddH2O. Aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul4C (-20C Glycerol)Blue IceRabbit
Concentration:
Not determined
Immunogen:
Synthetic PACAP-38 (BSA)
Purity:
Serum
Form
Supplied as a lyophilized powder. Reconstitute in 50-100ul sterile 40-50% glycerol, ddH2O. Dilute further in PBS, 1% BSA, 0.1% sodium azide.
Specificity:
Recognizes PACAP-38. Some crossreactivity with PACAP-27. Species Crossreactivity: Human, mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kato, J., et al., Acta Anat. 163: 92-98 (1998). 2. Hannibal, J., et al., Regulatory Peptides 55: 133-148 (1995). 3. Mikkelsen, J.D., et al., J. Neuroendocrinol. 7: 47-55 (1995) 4. Hamelink, C., et al., Proc. Natl. Acad. Sci. USA 99: 461-466 (2002).