		Technical Data
P1775-03	PACAP-38 (Pituitary Adenylate Cyclase Activating Polypeptide)

Description:
Pituitary adenylate cyclase activating peptide (PACAP), originally isolated from ovine hypothalamus, belongs to the VIP-family of peptides. It occurs in two biologically active forms, PACAP-38 and PACAP-27. PACAP is abundant in the brain, but can be also found in the respiratory and gastrointestinal tracts. Absorption with 10-100ug immunogen per ml diluted antiserum abolishes the staining, while PRP, VIP, PHI, CRF, oxytocin and vasopressin do not.

Applications:
Suitable for use in Immunofluorescence Microscopy and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunofluorescence Microscopy (Frozen sections): 1:400-1:800 with overnight incubation at 4°C.
Optimal dilutions to be determined by the researcher.

Positive Control:
Stefanini-fixed frozen sections of rat duodenum or hypothalamus.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Serum

Size		Storage	Shipping	Source	Host
50ul		4°C (-20°C Glycerol)	Blue Ice		Rabbit

Concentration:

Not determined

Immunogen:

Synthetic PACAP-38 (BSA)

Purity:

Serum

Form

Supplied as a lyophilized powder. Reconstitute in 50-100ul sterile 40-50% glycerol, ddH2O. Dilute further in PBS, 1% BSA, 0.1% sodium azide.

Specificity:

Recognizes PACAP-38. Some crossreactivity with PACAP-27. Species Crossreactivity: Human, mouse and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Kato, J., et al., Acta Anat. 163: 92-98 (1998). 2. Hannibal, J., et al., Regulatory Peptides 55: 133-148 (1995). 3. Mikkelsen, J.D., et al., J. Neuroendocrinol. 7: 47-55 (1995) 4. Hamelink, C., et al., Proc. Natl. Acad. Sci. USA 99: 461-466 (2002).