Technical Data
P3000-05
Pancreatic Polypeptide
Description:
Pancreatic polypeptide is found in the A cells of islet cell clusters of the exocrine pancreatic parenchyma and in the epithelium of small and medium-sized ducts, as well as focal acinar cells.

Applications:
Suitable for use in Immunohistochemistry, Immunofluorescence and Immunoperoxidase procedures. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Formalin-fixed and paraffin embedded sections of pancreatic tissue (6 microns thick) and cryostat sections of tissues can be used in the staining procedures.
Immunofluorescence: 1:20-1:40
Immunoperoxidase: 1:500-1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
500ul-20°CBlue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
Synthetic human PPP (active form) bound to keyhole limpet hemocyanin (KLH) with carbodiimide.
Purity:
Serum
Form
Supplied as a liquid, 0.1% sodium azide.
Specificity:
Recognizes human Pancreatic Polypeptide. Stains cells in the pancreas containing pancreatic polypeptide-like (PPP) immunoreactivity using both the immunoperoxidase and immunofluorescence procedures. Species Crossreactivity: sheep.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. R.C. Nairn, Fluorescent protein tracing, Livingstone, Edinburgh, 1964. 2. L.A. Sternberger, Immunocytochemistry, Wiley, New York 1979. 3. L.I. Larson et al. Diabetologia 12:211-226, 1976. 4. D. Baetens et al. Cell Tissue Res. 185:239-246, 1977. 5. J. Rahier et al. Cell Tissue Res. 200:359-366, 1979. 6. L.I. Larson et al. Cell Tissue Res. 156:167-171, 1975