Technical Data
PARP, Cleaved (Poly ADP-Ribose Polymerase)
The poly (ADP-ribose) polymerase (PARP) is involved in cell recovery from DNA damage, such as methylation of N3-adenine, which activates the base excision repair process. PARP [Poly (ADP-ribose) polymerase] is a 116kD nuclear chromatin-associated enzyme that is cleaved during apoptosis by caspase-3 into a 24kD fragment containing the DNA binding domain and an 89kD fragment containing the catalytic and automodification domains. The 24kD-fragment irreversibly bind to DNA and may contribute to the irreversibility of apoptosis by blocking the access of DNA repair enzymes to DNA strand breaks.

Suitable for use in Western Blot and Flow Cytometry. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml
Flow Cytometry (intracellular): 1-3ug per test
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG4H226Affinity Purified
100ug-20CBlue IceHumanMouse
Synthetic peptide containing amino acids near 214/215-cleavage site of human PARP.
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS containing 0.2% gelatin and 0.05% sodium azide.
Recognizes human PARP, Cleaved (Poly ADP-Ribose Polymerase). Specifically detects a 89-kDa cleavage fragment containing the catalytic and auto modification domain.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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