Technical Data
P3113-07J
PARP, Cleaved (Poly ADP-Ribose Polymerase)
Description:
The poly (ADP-ribose) polymerase (PARP) is involved in cell recovery from DNA damage, such as methylation of N3-adenine, which activates the base excision repair process. PARP [Poly (ADP-ribose) polymerase] is a 116kD nuclear chromatin-associated enzyme that is cleaved during apoptosis by caspase-3 into a 24kD fragment containing the DNA binding domain and an 89kD fragment containing the catalytic and automodification domains. The 24kD-fragment irreversibly bind to DNA and may contribute to the irreversibility of apoptosis by blocking the access of DNA repair enzymes to DNA strand breaks.

Applications:
Suitable for use in Western Blot and Flow Cytometry. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml
Flow Cytometry (intracellular): 1-3ug per test
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG4H226Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide containing amino acids near 214/215-cleavage site of human PARP.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS containing 0.2% gelatin and 0.05% sodium azide.
Specificity:
Recognizes human PARP, Cleaved (Poly ADP-Ribose Polymerase). Specifically detects a 89-kDa cleavage fragment containing the catalytic and auto modification domain.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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