Technical Data
Paxillin (PXN)
The cytoskeletal adaptor protein Paxillin is found at the interface between actin filaments and the plasma membrane. Paxillin localizes to focal adhesions, where it provides a platform for the integration and coordination of adhesion- and growth factor-related signals. Paxillin phosphorylation is required for integrin-mediated cytoskeletal reorganization, and may play a role in the disassembly of focal adhesions and stress fibers during cellular transformation. Increased Paxillin protein levels have also been reported in several malignancies, including renal, lung and breast carcinomas.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-61G: IgG H&L, X-adsorbed (HRP) Pab DkxSh
I1904-63: IgG H&L (HRP) Pab DkxSh

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS, 0.02% sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanSheep
Not determined
Partial recombinant human Paxillin, aa59-274 expressed in E. coli (P49023).
Purified by immunoaffinity chromatography.
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile PBS, 0.02% SODIUM AZIDE.
Recognizes human Paxillin. Species Crossreactivity: Mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.