Technical Data
P3113-90D
Paxillin (PXN)
Description:
Paxillin is a focal adhesion protein and a substrate for several tyrosine kinases such as src, FAK, and p120BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion, consistent with the belief that paxillin is involved in the regulation of cell morphology. Additionally because of is SH3-binding domain, paxillin associates tightly with FAK and Crk in a manner that is independent of the extracellular matrix. Although paxillin was initially detected in fibroblasts, its phosphorylation may also be important during neurite extension during differentiation.

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml
Immunohistochemistry (formalin fixed paraffin embedded): 1:50-1:100
Immunoprecipitation: 2ug/mg
Immunohistochemistry: Frozen
Optimal dilutions to be determined by the researcher.

Positive Control:
Colon carcinoma

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG111E312Ascites
SizeStorageShippingSourceHost
500ul-20CBlue IceHumanMouse
Concentration:
Not determined.
Immunogen:
Recombinant corresponding to full length, purified human paxillin. Cellular Localization: Cell membrane, cytoplasmic.
Purity:
Ascites
Form
Supplied as a liquid, 0.05% sodium azide.
Specificity:
Recognizes human Paxillin. Species Crossreactivity: bovine, avian, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. De Curtis & Malanchini Expt Cell Res 230: 233, 1997. 2. Hanks & Polte Bioessays 19: 137, 1997. 3. Leventhal et al. J Biol Chem 272: 5214, 1997.