Technical Data
Paxillin (PXN)
Paxillin is a focal adhesion protein and a substrate for several tyrosine kinases such as src, FAK, and p120BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion, consistent with the belief that paxillin is involved in the regulation of cell morphology. Additionally because of is SH3-binding domain, paxillin associates tightly with FAK and Crk in a manner that is independent of the extracellular matrix. Although paxillin was initially detected in fibroblasts, its phosphorylation may also be important during neurite extension during differentiation.

Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml
Immunohistochemistry (formalin fixed paraffin embedded): 1:50-1:100
Immunoprecipitation: 2ug/mg
Immunohistochemistry: Frozen
Optimal dilutions to be determined by the researcher.

Positive Control:
Colon carcinoma

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
500ul-20CBlue IceHumanMouse
Not determined.
Recombinant corresponding to full length, purified human paxillin. Cellular Localization: Cell membrane, cytoplasmic.
Supplied as a liquid, 0.05% sodium azide.
Recognizes human Paxillin. Species Crossreactivity: bovine, avian, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. De Curtis & Malanchini Expt Cell Res 230: 233, 1997. 2. Hanks & Polte Bioessays 19: 137, 1997. 3. Leventhal et al. J Biol Chem 272: 5214, 1997.