Technical Data
P3113-97P
PBEF (Pre-B cell Colony Enhancing Factor, Pre-B cell Enhancing Factor, 1110035O14Rik, DKFZP666B131, EC 2.4.2.12, MGC117256, Nicotinamide Phosphoribosyltransferase, NAmPRTase, NAMPT, Pre-B cell Colony Enhancing Factor 1, PBEF1, Visfatin)
Description:
Visfatin is pre-B cell colony-enhancing factor 1(PBEF), which is predominantly secreted from visceral adipose tissue both in humans and mice. This protein has also been reported to be a cytokine (PBEF) that promotes B cell maturation and inhibits neutrophil apoptosis, or a visceral-fat derived hormone that acts by binding and activating the insulin receptor.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:3000
Optimal dilutions to be determined by the researcher.

Hybridoma:
FO myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k8j11Affinity Purified
SizeStorageShippingSourceHost
50ul-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant corresponding toaa1-491 of human PBEF expressed in E. coli.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Specificity:
Recognizes human PBEF.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Stephens JM, et al., (2006) Curr Opin Lipidol. 17:128-31 2. Hug C, et al., (2005) Science. 307:36-97