		Technical Data
P3285-07	Peptide YY (PYY, Peptide Tyrosine Tyrosine)

Description:
The intestinal peptide YY is related to the PP-family of peptides and occurs in the glicentin cells in the gut. They are numerous in the rectum, colon and ileum and few in the duodenum and jejunum. PYY has hormone-like action, inhibits gut motility and pancreatic exocrine secretion and cause vasoconstriction. PYY may occur in endorine tumors of the pancreas and of the rectum.

Applications:
Suitable for use in Immunofluorescence Microscopy and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Frozen, paraffin): 1:1500-1:2500 using FITC, with overnight incubation at 4°C.
Optimal dilution to be determined by researcher.

Positive Control:
Frozen sections of rat intestine.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Serum

Size		Storage	Shipping	Source	Host
50ul		-20°C	Blue Ice		Guinea pig

Concentration:

Not determined

Immunogen:

Synthetic porcine peptide YY.

Purity:

Serum

Form

Supplied as a lyophilized powder. Reconstitute in 50-100ul sterile ddH20. Dilute further in 0.1M PBS, 1% BSA, 0.1% sodium azide.

Specificity:

Recognizes Peptide YY. Absorption with 10-100ug immunogen per ml diluted antiserum abolishes the staining. Species Crossreactivity: Human, rat, mouse, feline.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Portela-Gomes G et al: Colocalization of synaptophysin with different neuroendocrine hormones in the human gastrointestinal tract. Histochem Cell Biol 109, 281-291 (1998) 2. Myrsen-Axcrona U. et al. Developmental expression of NPY, PYY and PP in the rat pancreas and their coexistence with islet hormones. Regulatory peptides 68, 165-175 (1997) 3. Böttcher G et al. Peptide YY: intrapancreatic localization and effects on insulin and glucagon secretion in the mouse. Pancreas 4, 282-288 (1989)