		Technical Data
P3342-20D	Period Clock Protein 2 (Period 2, Period-2, Period 2 Isoform 1, Period Circadian Protein 2, Period Circadian Protein Homolog 2, Period Homolog 2, PER2, Circadian Clock Protein PERIOD2, Circadian Clock Protein PERIOD-2, FASPS, hPER2, KIAA0347)

Description:
PER2, a mammalian homolog of the Drosphilia period gene, shares 40% homology with PER1 including the protein dimerization PAS domain. A strong circadian rhythmic expression in the suprachiasmatic nuclei supports the view that the mammalian PER gene functions in the circadian clock with PER2 being a component of the circadian oscillator.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ul		4°C (-20°C Glycerol)	Blue Ice	Human	Rabbit

Concentration:

As reported

Immunogen:

Two peptides derived from human PER2. Antibodies crossreacting with each peptide were affinity purified against the appropriate peptide.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4.

Specificity:

Recognizes human PER2. Species Sequence Homology: Peptide 1 shows significant homology to rat (94.4%). Peptide 2 shows significant homology to rat and mouse (94.4%).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Shearman, L.P., et al., Neuron 19: 1261-1269 (1997). 2. Zheng, B., et al., Nature 400: 169-173 (1999). 3. Takumi, T., et al., Genes Cells. 3: 167-176 (1998). 4. Shearman, L.P. and Weaver, D.R., Neuroreport. 10: 613-618 (1999).