Technical Data
P3345-01
Peripherin (PRPH, Neurofilament 4, NEF4)
Description:
Peripherin is a 56-58kD type III intermediate filament protein, expressed in motor, sensory and sympathetic system neurons as well as neuroendocrine skin carcinomas and certain melanocytic tumors. It is often used an indicator of enteric ganglion cell density in normal and diseased (e.g. Hirschsprung's disease) states. Abnormal inclusions containing peripherin have also been reported in ALS. During normal, late neurogenesis peripherin appears after the expression of nestin, vimentin, alpha-internexin and neurofilaments and is involved in the differentiation of neurons, namely of the peripheral nervous system, and also probably in axonal regeneration. Peripherin is not related to peripherin-RDS, a photoreceptor protein associated with retinal degeneration and blindness.

Applications:
Suitable for use in Immunohistochemistry, Western Blot and Electron Microscopy. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry (Frozen, paraffin): 1:100-1:200 Fix frozen sections in acetone at -20°C. Works on tissue fixed for one hour or less in fresh 4% paraformaldehyde.
Western Blot: 1:1000 detected Peripherin on 10ug PC12 lysates. PC12 lysate was resolved by electrophoresis, transferred to PVDF membrance and probed with P3345-01. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.

Control:
Rat sensory neurons, rat spinal cord homogenate and peripheral nerve homogenate.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20°CBlue IceRatRabbit
Concentration:
Immunogen:
Electrophoretically pure trp-E-peripherin fusion protein containing all but the 4 N terminal amino acids of rat peripherin.
Purity:
Serum
Form
Supplied as a neat serum. No preservative added.
Specificity:
Recognizes rat Peripherin at ~57kD. Does not stain vimentin, GFAP, alpha-internexin or any of the neurofilament subunits. Species Crossreactivity: human, mouse, porcine and bovine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Dev. Neurosci. (1984) 6:335–344. 2. J. Neurosci. (1987) 7:781–791. 3. Neurofilament proteins. Pages 185–214 in “The Neuronal Cytoskeleton” Edited by Dr. R.D. Burgoyne, Alan Liss, New York (1991). 4. J. Neurosci. Research (1994) 37:515–528. 5. Somatosensory and Motor Research (1995) 12:49–57. 6. J. Neurocytology (1996) 25:181–196. 7. Cell & Tissue Research (1997) 288:11–23. 8. Kanitakis, J., et al., European J. Dermatology (1998) 8:339–342. 9. Hearing Research (1999) 133:149–154. 10. Liebl, D.J., et al., PNAS (1999) 97:2297–2302. 11. Kramer, P.R. et al., Mechanisms of Development (2000) 94:79–94. 12. Kramer, P.R. and Wray, S., Genes & Development (2000) 14:1824–1834.