Technical Data
P3345-02
Peripherin (PRPH, Neurofilament 4, NEF4)
Description:
Peripherin is a Class III intermediate filament subunit found in both the peripheral and central nervous systems, though it is concentrated, as its name suggests, in the neurons of peripheral ganglia and their processes. Antibodies to peripherin can be used in identifying, classifying, and studying neurons in the nervous system. Peripherin is also a good diagnostic marker for ballooned axons seen in Lou Gehrigs disease and some neuronally derived tumors. Peripherin is also a major component of the cytoskeleton of PC12 cells, widely used model system for studies of neuronal differentiation. Autoantibodies to peripherin are frequently seen in the sera of patients with diabetes. Peripherin is not related to peripherin-RDS, a photoreceptor protein associated with retinal degeneration and blindness.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceRatRabbit
Concentration:
Not determined
Immunogen:
Recombinant full length rat peripherin
Purity:
Serum
Form
Supplied as serum, 0.01% sodium azide
Specificity:
Recognizes peripherin, showing no crossreactivity with other Class III intermediate filament proteins. Species crossreactivity: Human and all other mammalian species to date. Results on avian tissues are however not conclusive
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Errante, L. et al., J. Neurosci. Res. 37:515-528 (1994).