Technical Data
P3345-04B
Peripherin (PRPH, PRPH1, Neurofilament 4, NEF4)
Description:
Perpherin is an intermediate filament protein expressed in peripheral neurons including enteric ganglian cells and it is present in neuronal derivatives of the neural crest. This antibody does not stain chromaffin cells, which is an advantage over other neural markers, but is a useful marker for neural crest derived tumors such as neuroblastomas and ganglioneuroblastomas for research applications.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:50-1:100 for 1 hr at RT
Immunohistochemistry: 1:100-1:200 for 1 hr at RT
Optimal dilutions to be determined by the researcher.

Positive Control:
Small bowel and neuroblastoma; peripheral nerves and ganglion cells (cytoplasmic staining pattern)

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2bPJM50Supernatant
SizeStorageShippingSourceHost
1ml-20CBlue IceMouse
Concentration:
Not determined
Immunogen:
Recombinant protein representing the complete peripherin molecule.
Purity:
Supernatant
Form
Supplied as a lyophilized powder in 15mM sodium azide. Reconstitute with 1ml dH2O.
Specificity:
Recognizes Peripherin. Species Crossreactivity: Human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Szabolcs M J, Visser J, Shelanski M L, et al.. Peripherin: A novel marker for the immunohistochemical study of malformations of the enteric nervous system. Pediatric Pathology & Laboratory Medicine. 16:51-70 (1996). 2.Foley J, Witte D, Chiu F-C, et al.. Expression of the neural intermediate filament proteins peripherin and neurofilament-66/a-internexin in neuroblas- toma. Laboratory Investigation. 71(2):193-199 (1994). 3.Kajiwara K, Berson E L and Dryja T P. Digenic retinitis pigmentosa due to mutations at the unlinked peripherin/rds and rom1 loci. Science. 264:1604-1608 (1994).