Technical Data
PHLPP (PH Domain and Leucine-rich Repeat Protein Phosphatase, PH Domain Leucine-rich Repeat Protein Phosphatase, PH Domain Leucine-rich Repeat-containing Protein Phosphatase, PHLPP1, KIAA0606 Protein, Pleckstrin Homology Domain-containing Family E Protein
PHLPPL (PH domain leucine-rich repeat-containing protein phosphatase-like) is responsible for the dephosphorylation and subsequent inactivation of Akt. Akt is a signaling protein critical to the balance of cell survival and apoptosis. Dephosphorylation of Akt by PHLPPL has been demonstrated to trigger apoptosis and suppress tumor cell growth. PHLPPL is also known as PHLPPL2 and functions similarly to its relative, PHLPP1. Recent studies show that PHLPPL and PHLPP selectively terminate Akt-signaling by targeting specific Akt isoforms. Alternate names for PHLPPL include PH Domain and Leucine Rich Repeat Protein Phosphatase-Like, KIAA0931, and PHLPP2.

Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:2000-1:10,000
Immunoprecipitation: 1-4ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ul-20CBlue IceHumanRabbit
Purified by immunoaffinity chromatography.
Supplied as a liquid in TBS, 0.1% BSA, 0.09% sodium azide.
Recognizes human PHLPP. The epitope recognized maps to a region between residues 1275 and the C-terminus (residue 1323) of human PH Domain and Leucine Rich Repeat Protein Phosphatase-like (NP_055835.2) (GeneID 23035). Species Crossreactivity: mouse, rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Mistafa et al., J Biol. Chem 285 (36):27900-27910 (2010)