		Technical Data
P4071-10E	Phosphatase, Alkaline, Intestine (Alkaline Phosphatase Intestinal, ALPI, Alkaline Phosphomonoesterase, Glycerophosphatase, Intestinal Alkaline Phosphatase, IAP, Intestinal Alkaline Phosphatase V, IAP-V, Kasahara Isozyme)

Description:
Alkaline phosphatase removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at a high pH. Most mammals have 4 different isozymes: placental, placental-like, intestinal and non-tissue specific (found in liver, kidney and bone). The antibody can be used as a second layer APAAP reagent for immunohistology.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	9A326	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Bovine	Mouse

Concentration:

~1mg/ml

Immunogen:

Calf intestinal phosphate. Cellular Localization: Attached to the membrane by a GPI-anchor.

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2. No preservative added.

Specificity:

Recognizes bovine Alkaline Phosphatase, Intestine.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

This product has been used in:Lai AP et al. Bone marrow macrophages and megakaryocytes express common acute lymphoblastic leukaemia antigen. Leuk Res 9:1155-9 (1985).