Technical Data
Phosphodiesterase 4B (PDE4B)
Of the 11 families of phosphodiesterase (PDEs) identified, PDE4, PDE7, and PDE8 are specific for cAMP. However, isoforms of the PDE4 family often account for most of the cAMP-hydrolyzing activity of a cell. Four genes (PDE4A, B, C and D) make up the PDE4 family. One or more subtype variants of PDE4B are ubiquitously present in all mammalian cells. In the CNS, PDE4B subtype variants are expressed in varying ratios. Peripheral tissues exhibit differential expression of PDE4B1, B2 and B4 variants.

Suitable for use in Immunoprecipitation and Western Blot. Other applications have not been tested.

Recommended Dilution;
Immunoprecipitation: 7ug/ml
Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
Synthetic peptide derived from the N-terminal region of human and rat PDE4B protein.
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Reacts with the human and rat PDE4B protein. On Western blots, it identifies a single band at ~83kD. Bands of unknown origin may be observed at ~160 and ~260kD. Reactivities have been confirmed with NG96 human neuroblastoma lysate and rat brain homogenate.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.