Technical Data
P4072-03E
Phosphodiesterase 4A (Phosphodiesterase 4A cAMP-specific, Phosphodiesterase-4A, PDE4A, cAMP Specific 3 5 Cyclic Phosphodiesterase 4A, cAMP Specific Phosphodiesterase, Cyclic AMP Phosphodiesterase PDE4A11, Cyclic AMP Specific Phosphodiesterase HSPDE4A10,
Description:
Cellular Localization: Cell Membrane

Applications:
Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to the C-terminal region of PDE4A (common to all PDE4A proteins).
Purity:
Purified by immunoaffinity chromatography.
Form
0.02% Thimerosal / Merthiolate
Specificity:
This antibody labels all known PDE4A variants including PDE4A1 (66kD), A5 (109kD), A8 (106kD), Ax (102kD) and a 76kD testis specific A variant. Species Crossreactivity: Human, Mouse, Rat and Rabbit.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Zhang HT et al. Inhibition of cyclic AMP phosphodiesterase (PDE4) reverses memory deficits associated with NMDA receptor antagonism. Neuropsychopharmacology 23:198-204 (2000). Yarwood SJ et al. The RACK1 signaling scaffold protein selectively interacts with the cAMP-specific phosphodiesterase PDE4D5 isoform. J Biol Chem 274:14909-17 (1999). Beavo JA Cyclic nucleotide phosphodiesterases: functional implications of multiple isoforms. Physiol Rev 75:725-48 (1995). Farooqui SM et al. Antibodies against synthetic peptides predicted from the nucleotide sequence of D2 receptor recognize native dopamine receptor protein in rat striatum. J Neurochem 57:1363-9 (1991).