Technical Data
P4072-03Y
Phosphodiesterase 4D (Phosphodiesterase 4D cAMP Specific, Phosphodiesterase-4D, PDE4D, cAMP Specific 3' 5' Cyclic Phosphodiesterase 4D, cAMP Specific Phosphodiesterase 4D, cAMP Specific Phosphodiesterase PDE4D, Cyclic AMP Phosphodiesterase, Dunce-like Pho
Description:
This gene encodes one of four mammalian counterparts to the fruit fly 'dunce' gene. The encoded protein has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP, which acts as a signal transduction molecule in multiple cell types. This gene uses different promoters to generate multiple alternatively spliced transcript variants that encode functional proteins.

Applications:
Suitable for use in ELISA and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Peptide ELISA Titer: 1:128,000
Western Blot: 0.1-0.3ug/ml ~70+80+90kD bands observed in lysates of cell line A549. In transfected COS1 transiently expressing PDE4D1 and PDE4D3 bands of ~68kD and 95kD are observed. These bands are not observed in the untransfected COS1. In transfected HEK293 transiently expressing PDE4D2 multi bands from ~55kD-80kD are observed. These bands are not observed in the non-transfected HEK293. Calculated MW of 91.1kD according to PDE4D1 (NP_001098101.1), 76.5kD according to PDE4D2 (NP_006194.2) and 84.7kD according to PDE4D3 (NP_001159371.1).
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to QPEACVIDDRSPDT, from the C Terminus of human Phosphodiesterase 4D (NP_001098101.1; NP_006194.2; NP_001159371.1).
Purity:
Purified by peptide affinity chromatography.
Form
Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity:
Recognizes human Phosphodiesterase 4D. Not expected to recognize isoform 4.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Houslay MD. The long and short of vascular smooth muscle phosphodiesterase-4 as a putative therapeutic target. Mol Pharmacol. 2005 Sep;68(3):563-7