Technical Data
P4072-06B1
Phosphodiesterase 6 beta (Phosphodiesterase 6B, Phosphodiesterase 6B cGMP-specific Rod beta, PDE6 beta, PDE6B, Congenital Stationary Night Blindness 3 Autosomal Dominant, CSNB3, Dunce-like Phosphodiesterase 4, DPDE4, GMP-PDE beta, PDEB, Rd1, Rod cGMP-spec
Description:
Photon absorption triggers a signaling cascade in rod photoreceptors that activates cGMP phosphodiesterase (PDE), resulting in the rapid hydrolysis of cGMP, closure of cGMP-gated cation channels and hyperpolarization of the cell. PDE is a peripheral membrane heterotrimeric enzyme made up of alpha, beta and gamma subunits. This gene encodes the beta subunit. Mutations in this gene result in retinitis pigmentosa and autosomal dominant congenital stationary night blindness. Multiple transcript variants encoding difference isoforms have been found for this gene.

Applications:
Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 2ug/ml
Immunohistochemistry: Frozen
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to aa20-36 of mouse and bovine PDE6 beta (HQYFG(K/R)KLSPENVAGAC). Cellular Localization: Plasma membrane
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 1mg/ml BSA, 0.05% sodium azide.
Specificity:
Recognizes mouse Phosphodiesterase 6 beta at ~90kD. Species Sequence Homology: bovine, human, canine
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
He F <i>et al.</i> Multiple zinc binding sites in retinal rod cGMP phosphodiesterase, PDE6alpha beta. <i>J Biol Chem</i> <b>275</b>:20572-7 (2000). <PUBMED:10787404>Granovsky AE & Artemyev NO Identification of the gamma subunit-interacting residues on photoreceptor cGMP phosphodiesterase, PDE6alpha '. <i>J Biol Chem</i> <b>275</b>:41258-62 (2000). <PUBMED:11024033>