Technical Data
P4155
Piericidin A (Shaoguanmycin B, Antibiotic MT 1882-I, Antibiotic SN 198E, Antibiotic IT 143D, Piericidin A1)
1mg
Biochemicals Storage: -20CShipping: Blue Ice
Piericidin A is the major analogue of a family of pyridyl antibiotics isolated from selected Streptomyces sp. It is a specific, potent inhibitor of NADH-ubiquinone oxidoreductase (Complex I) that binds to ubiquinone binding site(s). Piericidin A inhibits both mitochondrial and bacterial NADH-ubiquinone oxidoreductases, binding close to NUOD-NUOB interface.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

CAS Number:
2738-64-9

Molecular Formula:
C25H37NO4

Molecular Weight:
415.6
Source: Streptomyces sp.
Purity: ~95% by HPLC
Form: Supplied as a pale yellow oil. Soluble in ethanol, methanol, DMF or DMSO.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Evidence for a quinone binding site close to the interface between NUOD and NUOB subunits of
Complex I. Prieur I. et al. Biochim. Biophys. Acta 2001, 1504, 173.
2. -->H+/2e- stoichiometry in NADH-quinone reductase reactions catalyzed by bovine heart
submitochondrial particles. Galkin A.S. et al. FEBS Lett. 1999, 451, 157.
3. The 49-kDa subunit of NADH-ubiquinone oxidoreductase (Complex I) is involved in the binding of
piericidin and rotenone, two quinone-related inhibitors. Darrouzet E. et al. FEBS Lett. 1998, 10, 34.
4. Two binding sites of inhibitors in NADH: ubiquinone oxidoreductase (complex I). Relationship of one site
with the ubiquinone-binding site of bacterial glucose:ubiquinone oxidoreductase. Friedrich T. et al. Eur J
Biochem. 1994, 219, 691.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.