Platelet Derived Growth Factor AA, Recombinant, Human (PDGF-AA)
|Molecular Biology||Storage: -20°CShipping: RT|
PDGFs are disulfide-linked dimers consisting of two 12.0-13.5kD polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet alpha-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-alpha and PDGFR-beta. PDGFR-alpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-AA is a 28.5kD disulfide-linked homodimer of two A chains (250aa).F.
Determined by the dose-dependent stimulation of thymidine uptake by Balb/c 3T3 cells. The expected ED50 for this effect is 1ng/ml.
Concentration Range: 0.2-10ng/ml for most in vitro applications.
SIEEAVPAVC KTRTVIYEIP RSQVDPTSAN FLIWPPCVEV KRCTGCCNTS SVKCQPSRVH HRSVKVAKVE YVRKKPKLKE VQVRLEEHLE CACATTSLNP DYREEDTGRP RESGKKRKRK RLKPT
Storage and Stability:
Lyophilized powder may be stored at -20°C Stable for 12 months. Reconstitute with 10mM acetic acid. Aliquot and store at -20°C. Reconstituted product is stable for 3 months with a carrier protein. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Country of Origin: USA
P4258: Platelet Derived Growth Factor AA, Recombinant, Human (PDGF-AA)
P4258-01: Platelet Derived Growth Factor AA, Recombinant, Human (PDGF-AA)
P4258-01A: Platelet Derived Growth Factor AA, Recombinant, Human (PDGF-AA)
P4258-03: Platelet Derived Growth Factor AA, Recombinant, Human (PDGF-AA) Mab Mo xHu
P4258-04: Platelet Derived Growth Factor AA (PDGF-AA) Pab Rb xHu
P4258-04A: Platelet Derived Growth Factor AA (PDGF-AA) (Biotin) Pab Rb xHu
P4258-14: Platelet Derived Growth Factor AA (PDGF-AA) (Biotin) Pab Rb xHu
P4258-16A: Platelet Derived Growth Factor AA (PDGF-AA) Pab Rb xHu
P4258-19: Platelet Derived Growth Factor AA,AB,BB Neutralizing (PDGF-AA,AB,BB) Pab Gt xHu
Source: Human, recombinant expressed in E. coli
Purity: 98% by SDS-PAGE and HPLC Endotoxin: 0.1ng/ug (1EU/ug). Endotoxin: 0.1ng/ug.
Form: Supplied as a lyophilized powder.. Reconstitute with 10mM acetic acid to 0.1-1mg/ml. This solution can then be diluted into other aqueous solutions.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.