Technical Data
P4258
Platelet Derived Growth Factor AA, Recombinant, Human (PDGF-AA)
2ug
10ug
Molecular Biology Storage: -20CShipping: RT
PDGFs are disulfide-linked dimers consisting of two 12.0-13.5kD polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet alpha-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-alpha and PDGFR-beta. PDGFR-alpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-AA is a 28.5kD disulfide-linked homodimer of two A chains (250aa).F.

Biological Activity:
Determined by the dose-dependent stimulation of thymidine uptake by Balb/c 3T3 cells. The expected ED50 for this effect is 1ng/ml.

Concentration Range: 0.2-10ng/ml for most in vitro applications.

AA Sequence:
SIEEAVPAVC KTRTVIYEIP RSQVDPTSAN FLIWPPCVEV KRCTGCCNTS SVKCQPSRVH HRSVKVAKVE YVRKKPKLKE VQVRLEEHLE CACATTSLNP DYREEDTGRP RESGKKRKRK RLKPT

Storage and Stability:
Lyophilized powder may be stored at -20C Stable for 12 months. Reconstitute with 10mM acetic acid. Aliquot and store at -20C. Reconstituted product is stable for 3 months with a carrier protein. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Country of Origin: USA

Also Available:
P4258: Platelet Derived Growth Factor AA, Recombinant, Human (PDGF-AA)
P4258-01: Platelet Derived Growth Factor AA, Recombinant, Human (PDGF-AA)
P4258-01A: Platelet Derived Growth Factor AA, Recombinant, Human (PDGF-AA)
P4258-03: Platelet Derived Growth Factor AA, Recombinant, Human (PDGF-AA) Mab Mo xHu
P4258-04: Platelet Derived Growth Factor AA (PDGF-AA) Pab Rb xHu
P4258-04A: Platelet Derived Growth Factor AA (PDGF-AA) (Biotin) Pab Rb xHu
P4258-14: Platelet Derived Growth Factor AA (PDGF-AA) (Biotin) Pab Rb xHu
P4258-16A: Platelet Derived Growth Factor AA (PDGF-AA) Pab Rb xHu
P4258-19: Platelet Derived Growth Factor AA,AB,BB Neutralizing (PDGF-AA,AB,BB) Pab Gt xHu

Molecular Weight:
28.5kD
Source: Human, recombinant expressed in E. coli
Purity: 98% by SDS-PAGE and HPLC Endotoxin: 0.1ng/ug (1EU/ug). Endotoxin: 0.1ng/ug.
Form: Supplied as a lyophilized powder.. Reconstitute with 10mM acetic acid to 0.1-1mg/ml. This solution can then be diluted into other aqueous solutions.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Kaplan, M.R., et al., Differential Control of Clustering of the Sodium Channels Nav1.2 and Nav1.6 at Developing CNS Nodes of Ranvier, Neuron 30: 105-119 (2001). 2. Wang, S., et al., A Role for the Helix-Loop-Helix Protein Id2 in the Control of Oligodendrocyte Development, Neuron 29: 603-614 (2001). 3. Fernandez, P.A., et al., Evidence that Axon-Derived Neuregulin Promotes Oligodendrocyte Survival in the Developing Rat Optic Nerve, Neuron 28: 81-90 (2000). 4. Tang, D.G., et al., Long-Term Culture of Purified Postnatal Oligodendrocyte Precursor Cells: Evidence for an Intrinsic Maturation Program that Plays out over Months The Rockefeller University Press. The Journal of Cell Biology 148 (5 ): 971-984 (2000). 5. Hanson, M.G., et al., Cyclic AMP Elevation Is Sufficient to Promote the Survival of Spinal Motor Neurons In Vitro, The J. of Neuroscience 18 (18): 7361-7371 (1998). 6. Back, S.A., et al., Maturation-Dependent Vulnerability of Oligodendrocyted to Oxidative Stress-Induced Death Caused by Glutathione Depletion, The Journal of Neuroscience 18 (16): 6241-6253 (1998). 7. Fruttiger, M., et al., PDGF Mediates a Neuron-Astrocyte Interaction in the Developing Retina, Neuron 17: 1117-1131 (1996).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.