Technical Data
Platelet Derived Growth Factor A (PDGF-A) Chain, Recombinant, Human
Molecular Biology Storage: -20CShipping: Blue Ice
Recombinant PDGF-A comprises a 110 amino acid fragment (87-196) corresponding to the mature PDGF-A short chain protein and is expressed in E. coli with an amino-terminal hexahistidine tag.

The term PDGF refers to a family of disulfide bond- linked dimeric isoforms that act as autocrine and paracrine growth factors and are produced by a variety of cell types other than platelets. They act as potent mitogens for almost all mesenchymally-derived cells. Aberrant expression is involved in certain cancers, fibroproliferative disorders and atherosclerosis.

Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: Human, Recombinant, E. coli
Purity: Purified. 95% by SDS-PAGE.
Concentration: As reported
Form: Supplied as a liquid.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Raines, E.W. & Ross, R. (1982) J. Biol. Chem. 257(9) : 5154-5160.
Bonthron, D.T. et al. (1988). Proc. Natl. Acad. Sci USA 85(5):1492-1496. PDGF-A

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.