Technical Data
P4258-16B
Platelet Derived Growth Factor AA, Recombinant, Human (PDGF-AA)
10ug
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Technical Data |
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P4258-16B |
Platelet Derived Growth Factor AA, Recombinant, Human (PDGF-AA) |
10ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: Blue Ice |
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Human PDGF-AA homodimer is produced by genetically engineered yeast, and purified by ionic exchange chromatography and reverse-phase HPLC. This polypeptide is composed of two identical polypeptide chains attached by disulfide bonds. Biological Activity: Human PDGF-AA homodimer is fully active as measured by the mitogenic assay involving the stimulation of 3H-thymidine incorporation into NIH-3T3 cells. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute to with sterile dH2O or buffer, aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
Source: Yeast Purity: 95% pure by N-terminal aa sequencing, aa composition, HPLC analysis, and SDS gel electrophoresis. Concentration: As reported Form: Supplied as a lyophilized powder in PBS Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Heldin, C.H. and Westmark, B. (1990) Celll Regul. 1: 555-566 2. Raines, E. W. et al. (1990) Handbook of Experimental Pharmacology (Sporn, M.B. and Roberts, A.B. eds) Vol 9i5: pages 173-262 3. Ostman, A. et al. (1989) Growth Factors 1: 219-229
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