Technical Data
Platelet Derived Growth Factor AA, Recombinant, Human (PDGF-AA)
Molecular Biology Storage: -20CShipping: Blue Ice
Human PDGF-AA homodimer is produced by genetically engineered yeast, and purified by ionic exchange chromatography and reverse-phase HPLC. This polypeptide is composed of two identical polypeptide chains attached by disulfide bonds.

Biological Activity:
Human PDGF-AA homodimer is fully active as measured by the mitogenic assay involving the stimulation of 3H-thymidine incorporation into NIH-3T3 cells.

Storage and Stability:
Lyophilized powder may be stored at -20C. Reconstitute to with sterile dH2O or buffer, aliquot and store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: Yeast
Purity: 95% pure by N-terminal aa sequencing, aa composition, HPLC analysis, and SDS gel electrophoresis.
Concentration: As reported
Form: Supplied as a lyophilized powder in PBS

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Heldin, C.H. and Westmark, B. (1990) Celll Regul. 1: 555-566 2. Raines, E. W. et al. (1990) Handbook of Experimental Pharmacology (Sporn, M.B. and Roberts, A.B. eds) Vol 9i5: pages 173-262 3. Ostman, A. et al. (1989) Growth Factors 1: 219-229

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.