Technical Data
P4258-17M
Platelet Derived Growth Factor BB, Recombinant, Human (PDGF-BB, Platelet-derived Growth Factor B Chain, Platelet-derived Growth Factor beta Polypeptide, Platelet-derived Growth Factor Subunit B, PDGF Subunit B, PDGFB, Becaplermin, FLJ12858, PDGF2, PDGF-2,
10ug
Molecular Biology Storage: -20CShipping: Blue Ice
Source:
Recombinant corresponding to 109aa of human Platelet Derived Growth Factor-BB is a homodimeric, glycosylated, polypeptide chain.

Molecular Weight:
~32kD

Biological Activity:
The ED50, calculated by the dose-dependent proliferation of mouse BALB/c 3T3 indicator cells (measured by 3H-thymidine uptake).

Dimers and Aggregates:
1% (SDS-PAGE)

Protein Content:
Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280nm using the absorbency value of 0.55 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of PDGF-BB as a Reference Standard.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~32kD
Purity: ~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: 0.1ng/ug (IEU/ug)
Concentration: Not determined
Form: Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. ACEI attenuates the progression of CCl4-induced rat hepatic fibrogenesis by inhibiting TGF-beta1, PDGF-BB, NF-kappaB and MMP-2,9. World J Gastroenterol 2005 Aug 21;11(31):4807-11. 2. Luteolin prevents PDGF-BB-induced proliferation of vascular smooth muscle cells by inhibition of PDGF beta-receptor phosphorylation. Biochem Pharmacol 2005 Jun 15;69(12):1715-21. 3. PDGF-BB regulates p27 expression through ERK-dependent RNA turn-over in vascular smooth muscle cells. J Biol Chem 2005 Jul 8;280(27):25470-7. 4. Platelet-derived growth factor (PDGF-BB) and brain-derived neurotrophic factor (BDNF) induce striatal neurogenesis in adult rats with 6-hydroxydopamine lesions. Neuroscience 2005;132(3):767-76. 5. Platelet-derived growth factor-BB (PDGF-BB) induces differentiation of bone marrow endothelial progenitor cell-derived cell line TR-BME2 into mural cells, and changes the phenotype.
J Cell Physiol 2005 Sep;204(3):948-55. 6. Effects of separate application of three growth factors (TGF-beta1, EGF, and PDGF-BB) on mechanical properties of the in situ frozen-thawed anterior cruciate ligament. Clin Biomech (Bristol, Avon) 2005 Mar;20(3):283-90.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.