		Technical Data
P4268-01C	Plastin L, phosphorylated (Tyr28) (PLSL, L-Plastin, LC64P, Lymphocyte Cytosolic Protein 1, LCP1, LCP-1, Plastin 2, Plastin-2)

Description:
Highly conserved and widely expressed plastin proteins comprise a subset of actin-binding proteins that include proteins that promote actin bundling. Three plastins exhibiting differential expression are found in mam-mals and include L-plastin, T-plastin and I-plastin. T-plastin
(plastin-3) is found in cells of most solid tissues while I-plastin (plastin-1) is expressed specifically in kidney, colon and small intestine (1-3). L-plastin (plastin-2) or LCP1 (lymphocyte cytosolic protein 1) is mainly expressed in he-matopoietic cells and nonhematopoietic tumors, including increased expression correlated with metastatic progression in colon cancer cell lines (4). Overexpression of LCP1 in premetastatic cancer cell lines induces invasion and loss of
E-cadherin expression, which is characteristic of metastatic cancer cell lines (5). LCP1 becomes phosphorylated at Ser5 upon stimulation through the T-cell receptor/co-receptor complex (TCR/CD3) in association with the CD2 cell adhe-sion molecule or the CD28 receptor (6). Phosphorylation at Ser5 increases LCP1 ability to bind F-actin and increase cell
motility (7,8).

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

Not determined

Immunogen:

Synthetic phosphopeptide corresponding to residues surrounding Tyr28 of human LCP1protein (KLH-coupled).

Purity:

Purified by Protein A affinity chromatography

Form

Supplied as a liquid in 10mM sodium HEPES pH 7.5, 0.1%, 150mM NaCl, 100ug/ml BSA and 50% glycerol.

Specificity:

Recongnizes endogenous levels of human LCP1 protein only when phosphorylated on Tyr28.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Lin, C.S. et al. (1993) J Biol Chem 268, 2781-92, 2. Lin, C.S. et al. (1994) Mol Cell Biol 14, 2457-67, 3. Delanote, V. et al. (2005) Acta Pharmacol Sin 26, 769-79, 4. Otsuka, M. et al. (2001) Biochem Biophys Res Commun 289, 876-81, 5. Foran, E. et al. (2006) Int J Cancer 118, 2098-104, 6. Wabnitz, G.H. et al. (2007) Eur J Immunol 37, 649-62, 7. Janji, B. et al. (2006) J Cell Sci 119, 1947-60, 8. Klemke, M. et al. (2007) Int J Cancer 120, 2590-9, 9. Rush, J. et al. (2005) Nat Biotechnol 23, 94-101, 10. Rikova, K. et al. (2007) Cell 131, 1190-203, 11. Gu, T.L. et al. (2007) Blood 110, 323-33, 12.Walters, D.K. et al. (2006) Leuk Res 30, 1097-104.