Technical Data
P5640-15D
PRAS40 (Proline-Rich AKT Substrate 40kD, 40kD Proline-Rich AKT Substrate, AKT1 Substrate 1 (Proline-rich), AKT1 Substrate 1, AKT1S1, Lobe) (Alexa FluorŪ488)
Description:
Proline-Rich Akt Substrate (PRAS40) is a 40kD substrate of Akt. Akt and PRAS40 can be found in the phosphoinositide 3-kinase (PI3K) pathway, which plays a role in glucose uptake, cell growth, and apoptosis inhibition. PRAS40 is a 14-3-3 binding protein that reacts with insulin, but whose precise function is not yet known. It has been hypothesized that it may play a role in interacting with SH3 and WW domain containing proteins. The binding of PRAS40 to these proteins may bring about a change of function. Activated Akt phosphorylates PRAS40 on threonine 246, which is thereby able to bind 14-3-3. Mutation of PRAS40 threonine 246 has been shown to be apoptotic, and plays a role in abrogating neuronal protection.

Applications:
Suitable for use in Immunofluorescence. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:25.
Optimal dilutions to be determined by the researcher.

Positive Control: NIH3T3 cells.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Light sensitive.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul4°C (-20°C Glycerol)Blue IceHumanRabbit
Concentration:
Not determined.
Immunogen:
Synthetic phosphopeptide corresponding to the region of human PRAS40 that contains threonine 246. Cellular Localization: Nuclear.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 0.05% sodium azide 0.2% BSA (without Mg2+ and Ca2+), pH 7.3. Labeled with Alexa FluorŪ488.
Specificity:
Recognizes human PRAS40. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kovacina, K.S., et al. (2003) Identification of a proline-rich Akt substrate as a 14-3-3 binding partner. J. Biol. Chem. 278(12):10189-10194. 2. Cantley, L.C. (2002) The phosphoinositide 3-kinase pathway. Science. 296(5573):1655-1657. 3. Lizcano, J.M. and D.R. Alessi (2002) The insulin signaling pathway. Curr. Biol. 12(7):R236-R238. C4. ho, H., et al. (2001) Akt1/PKBalpha is required for normal growth but dispensable for maintenance of glucose homeostasis in mice. J. Biol. Chem. 276(42):38349-38352.