Technical Data
PRAS40 (Proline-Rich AKT Substrate 40kD, 40kD Proline-Rich AKT Substrate, AKT1 Substrate 1 (Proline-rich), AKT1 Substrate 1, AKT1S1, Lobe) (Alexa FluorŪ488)
Proline-Rich Akt Substrate (PRAS40) is a 40kD substrate of Akt. Akt and PRAS40 can be found in the phosphoinositide 3-kinase (PI3K) pathway, which plays a role in glucose uptake, cell growth, and apoptosis inhibition. PRAS40 is a 14-3-3 binding protein that reacts with insulin, but whose precise function is not yet known. It has been hypothesized that it may play a role in interacting with SH3 and WW domain containing proteins. The binding of PRAS40 to these proteins may bring about a change of function. Activated Akt phosphorylates PRAS40 on threonine 246, which is thereby able to bind 14-3-3. Mutation of PRAS40 threonine 246 has been shown to be apoptotic, and plays a role in abrogating neuronal protection.

Suitable for use in Immunofluorescence. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:25.
Optimal dilutions to be determined by the researcher.

Positive Control: NIH3T3 cells.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Light sensitive.
PabIgGAffinity Purified
100ul4°C (-20°C Glycerol)Blue IceHumanRabbit
Not determined.
Synthetic phosphopeptide corresponding to the region of human PRAS40 that contains threonine 246. Cellular Localization: Nuclear.
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, 0.05% sodium azide 0.2% BSA (without Mg2+ and Ca2+), pH 7.3. Labeled with Alexa FluorŪ488.
Recognizes human PRAS40. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kovacina, K.S., et al. (2003) Identification of a proline-rich Akt substrate as a 14-3-3 binding partner. J. Biol. Chem. 278(12):10189-10194. 2. Cantley, L.C. (2002) The phosphoinositide 3-kinase pathway. Science. 296(5573):1655-1657. 3. Lizcano, J.M. and D.R. Alessi (2002) The insulin signaling pathway. Curr. Biol. 12(7):R236-R238. C4. ho, H., et al. (2001) Akt1/PKBalpha is required for normal growth but dispensable for maintenance of glucose homeostasis in mice. J. Biol. Chem. 276(42):38349-38352.