Technical Data
P6300-40A
Presenilin 2
Description:
Presenilin 1 and presenilin 2 are transmembrane proteins belonging to the presenilin family. Mutation of presenilin genes has been linked to early onset of Alzheimer’s disease, probably functioning through its associated gamma-secretase activity for amyloid-beta protein processing (1,2). Endogenous presenilin mainly exists in a heterodimeric complex formed from the endoproteolytically processed N-terminal (34kD) and C-terminal (~20kD) fragments (2,3).

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:1000
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide corresponding to residues surrounding aa330 of human presenilin 2.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity:
Recognizes endogenous levels of the carboxy-terminal fragment of human presenilin 2 (~20kD) and the full length protein (54kD) to a lesser extent. It does not crossreact with endogenous levels of presenilin 1. Species Crossreactivity: monkey, mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Haass, C. and DeStrooper, B. (1999) Science 286, 916–919. 2. Kimberly, W.T. et al. (2000) J. Biol. Chem. 275, 3173–3178. 3. Kim, T.W. et al. (1997) J. Biol. Chem. 272, 11006–11010. 4. Hashimoto, Y. et al. (2002) J. Pharma. Exp. Thera. 300(3), 736–745.