Technical Data
Presenilin 2
Presenilin 1 and presenilin 2 are transmembrane proteins belonging to the presenilin family. Mutation of presenilin genes has been linked to early onset of Alzheimer’s disease, probably functioning through its associated gamma-secretase activity for amyloid-beta protein processing (1,2). Endogenous presenilin mainly exists in a heterodimeric complex formed from the endoproteolytically processed N-terminal (34kD) and C-terminal (~20kD) fragments (2,3).

Suitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:1000
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.

Positive Control:
293 and HeLa (human), NIH/3T3 and L929 (mouse), and C6 and PC12 (rat) cell lines

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ul-20°CBlue IceHumanRabbit
Not determined
Synthetic peptide corresponding to residues surrounding aa330 of human presenilin 2.
Purified by Protein A and immunoaffinity chromatography.
Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Recognizes endogenous levels of the carboxy-terminal fragment of human presenilin 2 (~20kD) and the full length protein (54kD) to a lesser extent. Does not crossreact with endogenous levels of presenilin 1. Species Crossreactivity: monkey, mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Haass, C. and DeStrooper, B. (1999) Science 286, 916–919. 2. Kimberly, W.T. et al. (2000) J. Biol. Chem. 275, 3173–3178. 3. Kim, T.W. et al. (1997) J. Biol. Chem. 272, 11006–11010. 4. Hashimoto, Y. et al. (2002) J. Pharma. Exp. Thera. 300(3), 736–745.