Technical Data
Prolyl Hydroxylase 4, beta (P4HB, Cellular thyroid hormone-binding protein, DSI, ERBA2L, GIT, P4Hbeta, p55, PDI, PDIA1, PHDB, PO4DB, PO4HB, PROHB, Prolyl 4-hydroxylase subunit beta, Protein disulfide-isomerase)
Prolyl hydroxylase 4 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the prolyl hydroxylase sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. Prolyl hydroxylase is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, prolyl hydroxylases convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis. Prolyl hydroxylase 4, specifically, catalyzes the post-translational formation of 4-hydroxyproline in HIF alpha proteins. It may function as a cellular oxygen sensor and, under normoxic conditions, may targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex.

Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 2.5ug/ml
Immunohistochemistry (Frozen and paraffin-embedded tissue sections): 20ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ug-20CBlue IceHumanMouse
Human prolyl 4-hydroxylase (hPH).
Supplied as a liquid in PBS, pH 7.0, 2% BSA (Protease free).
Recognizes the b-subunit of human Prolyl Hydroxylase, 4.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bai, Y. et al. Immunological properties of monoclonal antibodies to human and rat prolyl 4-hydroxylase. J. Biochem., 99, 1563-1570, 1986. 2. Yoshida, S. et al. A sandwich immunoassay for human propyl 4-hydroxylase using monoclonal antibodies. Clin.Clim. Acta, 160, 37-46, 1986.