Technical Data
P9052-40A
Proprotein Convertase Subtilisin Kexin 9 (Proprotein Convertase Subtilisin/kexin Type 9, PCSK9, FH3, Hypercholesterolemia Autosomal Dominant 3, HCHOLA3, Neural Apoptosis Regulated Convertase 1, NARC1, NARC-1, PC9, Proprotein Convertase PC9, PSEC0052, Subt
Description:
This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. The encoded protein is synthesized as a soluble zymogen that undergoes autocatalytic intramolecular processing in the endoplasmic reticulum. The protein may function as a proprotein convertase. This protein plays a role in cholesterol homeostasis and may have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a third form of autosomal dominant familial hypercholesterolemia (HCHOLA3).

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1600
Western Blot: 0.3ug/ml. In McA cell lysates, two bands are observed at ~73 and 60kD representing unprocessed and processed protein.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide CRSRHLAQASQELQ, representing the C-Terminus of the human protein sequence according to NP_777596.2.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris-saline, pH 7.2. 0.5% BSA, 0.02% sodium azide.
Specificity:
Recognizes human PCSK9.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Cohen J, Pertsemlidis A, Kotowski IK, Graham R, Garcia CK, Hobbs HH. Low LDL cholesterol in individuals of African descent resulting from frequent nonsense mutations in PCSK9. Nat Genet. 2005 Feb;37(2):161-165. Epub 2005 Jan 16. PMID: 15654334