Technical Data
P9078-38H
PSMB9, CT (Proteasome Subunit beta Type-9, Proteasome Subunit beta-1i, Proteasome Chain 7, Macropain Chain 7, Multicatalytic Endopeptidase Complex Chain 7, Really Interesting New Gene 12 Protein, RING12 Protein, Low Molecular Mass Protein 2, RING12, LMP2)
Description:
PSMB9 is a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides.

Applications:
Suitable for use in ELISA, Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:10-1:50
Western Blot: 1:1000
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 193-219 in the C-terminal region of human PSMB9, UniProt Accession #P28065.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human PSMB9.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Honcharov,S.V., et.al., Fiziol Zh 55(2), 3-10 (2009). 2. Moschonas,A., et.al., Mol. Cell. Biol. 28(20), 6208-6222 (2008)