		Technical Data
P9104-91	Podoplanin (PDPN, Aggrus, Glycoprotein 36kD, Glycoprotein 36, gp36, GP38, HT1A-1, hT1alpha1, hT1alpha2, Lung Type-I Cell Membrane-associated Glycoprotein Isoform a, OTS8, OTS-8, PA2.26 Antigen, T1 alpha, T1A, TIA2)

Description:
Podoplanin is absent on blood endothelium and therefore this product is readily able to distinguish between lymphatic vessels and blood vasculature. Although the specific function of this protein is undetermined, Podoplanin is reported to be a reliable indicator of tumor lymphangiogenesis.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Frozen and paraffin): 0.75-2ug/ml
Note: Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Use sodium citrate buffer, pH 6.0.
Optimal dilution determined by the researcher.

Recommended Secondary Reagents:
I1904-65W: IgG, F(ab’)2 (HRP) Pab RbxMo
I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab RbxMo

Recommended Negative Control:
I1904-78R: IgG1 Murine Negative Control

Recommended Positive Control Tissue:
Normal lung

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	6A457	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

ORF Human podoplanin ectodomaine expressed in E. coli.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

Specificity:

Recognizes Human Podoplanin (gp36), a 36kD type I cell membrane-associated glycoprotein, with a broad spectrum of distribution, being strongly expressed on the lymphatic endothelium found in lung, placenta and skeletal muscle and to a lesser extent in brain, kidney and liver.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Matsui, K. et al. (2003). J. Am. Soc. Nephrol. 14: 1981-1989.2. Dadras, S. S. et al. (2003). Am. J. Pathol. 162: 1951-1960.3. Zimmer, G. et al. (1999). Biochem J. 341: 277-284.4. Schacht, V. et al. (2003). EMBO J. 22: 3546-3556.5. Kato, Y. et al. (2003). Molecular Identification of Aggrus/T1alpha as a Platelet Aggregation-inducing Factor Expressed in Colorectal Tumors. J. Biol Chem 278: 51599-51605.