Technical Data
P9107-34M
PPP2CA, phosphorylated, Tyr307 (Protein Phosphatase 2, catalytic subunit, alpha isozyme, PP2A-alpha, PP2Ac, PP2CA, PP2Calpha, Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform)
Description:
Protein phosphatase type 2A (PP2A) is a protein serine/threonine phosphatase that controls fundamental cellular processes, including transcription, translation, metabolism, cell growth, apoptosis, and varied other signal transduction pathways. PP2A is comprised of a core enzyme, which is made up of catalytic C and regulatory A (PR65) subunits, and a secondary regulatory B subunit. This B subunit contains four different protein families. In response to EGF or insulin, it has been shown that Src phosphorylates PP2A at Tyr307, thereby inactivating PP2A.

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:5000
Immunohistochemistry: 1:50
Immunocytochemistry: 1:50
Immunoprecipitation: 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG6k333Supernatant
SizeStorageShippingSourceHost
100ul 4°C (-20°C Glycerol)Blue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
A synthetic phospho-peptide corresponding to residues surrounding Tyr307 of human PP2A
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
Specificity:
Recognizes human PP2A phosphorylated on Tyrosine 307. MW: 36kD. Species crossreactivity: rat. Species sequence homology: bovine and porcine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Janssens, V. and J. Goris. Protein phosphatase 2A: a highly regulated family of serine/threonine phosphatases implicated in cell growth and signalling. Biochem. J. 353: 417–439 (2001). 2. Zabrocki, P., et al. Protein phosphatase 2A on track for nutrient-induced signalling in yeast. Mol. Microbiol. 43: 835–842 (2002). 3. Milward, T.A., et al. Regulation of protein kinase cascades by protein phosphatase 2A. Trends Biochem. Sci. 24: 186–191 (1999). 4. Chen, J., et al. Regulation of protein serine-threonine phosphatase type-2A by tyrosine phosphorylation. Science 257: 1261–1264 (1992).