Technical Data
P9107-34M
PPP2CA, phosphorylated, Tyr307 (Protein Phosphatase 2, catalytic subunit, alpha isozyme, PP2A-alpha, PP2Ac, PP2CA, PP2Calpha, Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform)
Description:
Protein phosphatase type 2A (PP2A) is a protein serine/threonine phosphatase that controls fundamental cellular processes, including transcription, translation, metabolism, cell growth, apoptosis, and varied other signal transduction pathways. PP2A is comprised of a core enzyme, which is made up of catalytic C and regulatory A (PR65) subunits, and a secondary regulatory B subunit. This B subunit contains four different protein families. In response to EGF or insulin, it has been shown that Src phosphorylates PP2A at Tyr307, thereby inactivating PP2A.

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:5000
Immunohistochemistry: 1:50
Immunocytochemistry: 1:50
Immunoprecipitation: 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG6k333Supernatant
SizeStorageShippingSourceHost
100ul 4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
A synthetic phospho-peptide corresponding to residues surrounding Tyr307 of human PP2A
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
Specificity:
Recognizes human PP2A phosphorylated on Tyrosine 307. MW: 36kD. Species crossreactivity: rat. Species sequence homology: bovine and porcine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Janssens, V. and J. Goris. Protein phosphatase 2A: a highly regulated family of serine/threonine phosphatases implicated in cell growth and signalling. Biochem. J. 353: 417439 (2001). 2. Zabrocki, P., et al. Protein phosphatase 2A on track for nutrient-induced signalling in yeast. Mol. Microbiol. 43: 835842 (2002). 3. Milward, T.A., et al. Regulation of protein kinase cascades by protein phosphatase 2A. Trends Biochem. Sci. 24: 186191 (1999). 4. Chen, J., et al. Regulation of protein serine-threonine phosphatase type-2A by tyrosine phosphorylation. Science 257: 12611264 (1992).