Technical Data
Media
Pseudomonas cepacia Agar (Powder)
P9175-02
Microbiological Grade
Storage RT    Shipping RT
Formulation per Liter:
Componentg/L
Enzymatic Digest of Animal Tissue1.0
Sodium Pyruvate5.0
Bile Extract1.5
Ammonium Sulfate1.0
Ferrous Ammonium Sulfate0.01
Magnesium Sulfate0.2
Dipotassium Phosphate4.3
Monopotassium Phosphate 2.1
Phenol Red0.02
Crystal Violet0.001
Agar15.0
Final pH: 7.1 0.2 at 25C
Supplement 10ml/Liter
Ticarcillin (100mg)
Polymyxin B (300,000U)
Formula may be adjusted and/or supplemented as required to meet performance specifications.
Selective medium for isolation Pseudomonas cepacia (Burkholderia
cepacia) from sputum samples of cystic fibrosis patients.

Dehydrated Appearance:
Powder is homogeneous, free flowing, and light beige.

Prepared Appearance:
Prepared medium is trace to slight hazy and peach.

Expected Cultural Response:
Cultural response on Pseudomonas Cepacia Agar at 35C after 18-48 hours incubation.

Storage:
Store sealed bottle containing the dehydrated medium at 2-30C. Once opened and recapped, place container in a low humidity environment at the same storage temperature. Protect from moisture and light by keeping container tightly closed.

Expiration:
12 months after receipt of order. The dehydrated medium should be discarded if not free flowing, or if the appearance has changed from the original color. Expiry applies to medium in its intact container when stored as directed.





1. Suspend 30g of the medium in one liter of purified water. 2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.3. Autoclave at 121C for 15 minutes.

4. Cool to 45-50C and aseptically add a filter sterilized solution containing Ticarcillin (100mg) and Polymyxin B (300,000 U) dissolved in 10ml of sterile water.Test Procedure:1. Inoculate medium using the streak plate method to obtain isolated colonies.2. Incubate for 18-72 hours at 35C.Results:Examine for presence of growth. Pseudomonas cepacia colonies will be lime green, and the medium surrounding the colonies will be pink to hot-pink in color.

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Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Gilardi, G. L. 1991. In A. Balows, W. J. Hausler, Jr., K. L. Herrmann, H. D. Isenberg, and H. J. Shadomy (eds.) Manual of clinical microbiology, 5th ed. American Society of Microbiology, Washington, D.C.
2. Iglewski, B. H. 2001. Pseudomonas. In S. Baron (ed). Medical Microbiology. University of Texas Medical Branch, Galveston, TX.
3. Chaparro, C., J. Maurer, C. Gutierrez, M. Krajden, C. Chan, T. Winton, S. Keshavjee, M. Scavuzzo, E. Tullis, M. Hutcheon, and S. Kesten, 2001. Infection with Burkholderia cepacia in Cystic Fibrosis. Am. J. Respir. Crit. Care Med. 163:43-48.
4. Gilligan, P. H., et al. 1985. In P. R. Murray, E. J. Baron, M. A. Pfaller, F. C. Tenover, and R. H. Yolken (eds.). Manual of clinical microbiology, 4th ed. American Society of Microbiology, Washington, D.C.