Technical Data
Pseudomonas Isolation Agar (Powder)
Microbiological Grade
Storage RT/4C    Shipping RT
Formulation g/L:
Magnesium Chloride1.4
Potassium Sulfate10.0
Total: 45.03g/L
Pseudomonas Isolation Agar is a modification of a formula described by King et al. Pseudomonas Isolation Agar was found useful in the isolation of Pseudomonas from pharmaceutical preparations (lotions and creams). The incorporation of lrgasan (R) inhibits most bacteria but allows the growth of Pseudomonas. The formulation also enhances pyocyanin production.

Directions per Liter: Dissolve 45.03 grams in 800-900ml of ddH2O containing 20ml of glycerol, stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. If required, add sodium bicarbonate. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi).

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

Appearance: Off-white to tan, homogeneous, free flowing powder

Solubility: Light yellow to tan, clear, complete with heat

pH: As Reported

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.