Technical Data
The rabies virus is thetype speciesof theLyssavirusgenus, in the familyRhabdoviridae, orderMononegavirales. Lyssaviruses have helical symmetry, with a length of about 180nmand a cross-section of about 75nm.These viruses areenvelopedand have a single-strandedRNAgenome withnegative sense. The genetic information is packed as aribonucleoproteincomplex in which RNAis tightly bound by the viral nucleoprotein. TheRNA genomeof the virus encodes five genes whose order is highly conserved: nucleoprotein (N), phosphoprotein (P), matrix protein (M), glycoprotein (G),and the viral RNA polymerase (L).

Once within a muscle or nerve cell, the virus undergoes replication. The trimeric spikes on the exterior of the membrane of the virus interact with a specific cell receptor, the most likely one being theacetylcholine receptor. The cellular membrane pinches in a procession known aspinocytosisand allows entry of the virus into the cell by way of an endosome. The virus then uses the acidicenvironment of that endosome and binds to its membrane simultaneously, releasing its five proteins and single strand RNA into the cytoplasm.

The L protein then transcribes five mRNA strands and a positive strand of RNA all from the original negative strand RNA using free nucleotides in the cytoplasm. These five mRNA strands are thentranslated into their corresponding proteins (P, L, N, G and M proteins) at free ribosomes in the cytoplasm. Some proteins require post-translative modifications. For example, the G protein travelsthrough the roughendoplasmic reticulum, where it undergoes further folding, and is then transported to theGolgi apparatus, where a sugar group is added to it (glycosylation).

Where there are enough proteins, the viral polymerase will begin to synthesize new negative strands of RNA from the template of the positive strand RNA. These negative strands will then formcomplexes with the N, P, L and M proteins and then travel to the inner membrane of the cell, where a G protein has embedded itself in the membrane. The G protein then coils around the N-P-L-Mcomplex of proteins taking some of the host cell membrane with it, which will form the new outer envelope of the virus particle. The virus then buds from the cell.From the point of entry, the virus isneurotropic, traveling quickly along the neural pathways into thecentral nervous system, and then to other organs.Thesalivary glandsreceive high concentrationsof the virus, thus allowing further transmission.

Suitable for use in Immunofluorescence, Indirect ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Formalin-fixed paraffin embedded sections.
Optimal dilutions to be determined by the researcher.

Immunofluorescence: >1x10e3 (Titer by Native BHK)
Indirect ELISA: 1.5x10e6 (Titer by Indirect ELISA)

Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG2a1.B.456Affinity Purified
1mg-20CBlue IceMouse
Purified rabies virus
Purified by Protein G chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Recognizes a glycoprotein of more than 20 different strains of rabies virus from 4 serogroups: CVS, Lagosbat, Mokola, Duwenhage were positive in neutralization reaction.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Macikova, I., et al., Common and different antigenic properties of the rabies virus glycoprotein of strains SAD-Vnukovo and Pitman-Moore. Acta. Virol. 36(6): 541-550 (1992).
2. Drew WL (2004). "Chapter 41: Rabies". In Ryan KJ, Ray CG (editors). Sherris Medical Microbiology (4th ed.). McGraw Hill. pp. 597600. ISBN 0-8385-8529-9.
3. Finke S, Conzelmann KK (August 2005). "Replication strategies of rabies virus". Virus Res. 111 (2): 12031. doi:10.1016/j.virusres.2005.04.004. PMID 15885837.