Technical Data
R1031-15
RAGE (Receptor for Advanced Glycosylation End Products, Advanced Glycosylation End Product-specific Receptor, AGER)
Description:
RAGE is a member of the immunoglobulin superfamily of cell surface molecules that bind molecules which have been irreversibly modified by non-enzymatic glycation and oxidation, and are known as advanced glycation end products (AGEs). RAGE is expressed by endothelium, mononuclear phagocytes, neurons and smooth muscle cells. Whereas RAGE is present at high levels during development, especially in the central nervous system, its levels decline during maturity. The increased expression of RAGE is associated with several pathological states, such as diabetic vasculopathy, neuropathy, retinopathy and other disorders, including Alzheimer’s disease and immune/inflammatory reactions of the vessel walls. In diabetic tissues, the production of RAGE is due to the overproduction of AGEs that eventually overwhelm the protective properties of RAGE. This results in oxidative stress and endothelial cell dysfunction that leads to vascular disease in diabetics. In the brain, RAGE also binds amyloid beta (A beta). Because A beta is overproduced in neurons and vessels in the brains of Alzheimer’s disease, this leads to the hyperstimulation of RAGE. The RAGE-A beta interaction is thought to result in oxidative stress leading to neuronal degeneration.

Applications:
Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and ELISA. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:500 on 10ug of mouse brain lysates. Detects a band at ~48kD.
Immunohistochemistry: Paraffin embedded tissue sections.
Immunocytochemistry: Cells fixed with ice cold acetone using a secondary antibody labeled with Cy3.
ELISA: Direct
Optimal dilutions to be determined by the researcher.

Recommended Control:
Lung tissue and mouse brain lysate

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a2Q96Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant truncated extracellular portion of human RAGE expressed in E. coli.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% sodium azide.
Specificity:
Recognizes both natural and recombinant human RAGE. Species Crossreactivity: bovine and mouse. Species Sequence Homology: rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Srikrishna, G., et al., J. Neurochem. 80: 988-1008 (2002). 2. Bierhaus, A., et al., Diabetes 50: 2792-2808 (2001). 3. Chou, D.K.H., et al., J. Neurochem. 90(6): 1389-1401 (2004). 4. Fiuza, C., et al., Blood 101(7): 2652-2660 (2003). 5. Most, P., et al., J. Biol. Chem. 278: 48,404-48,412 (2003). 6. Nakamura, S., et al., Am. J. Kidney Dis. 41(3 Suppl 1): S61-S67 (2003). 7. Rocken, C., et al., Am. J. Kidney Dis. 162(4): 1213-1220 (2003). 8. Ponath, G., et al., J. Neuroimmunol. 184: 214-222 (2007).