		Technical Data
R1075	RANK, Recombinant, Human (Receptor Activator of NFkB)	2ug 10ug

Growth Factors, Cytokines

Storage: 4°C/-20°CShipping: Blue Ice

Human RANK (receptor activator of NF-KappaB), a soluble 20kD protein, comprising the full-length of the TNF-like extracellular domain of RANKL, is a member of the TNFR family, identified as a dentritic cell membrane protein.

Endotoxin:
0.1ng/ug (1EU/ug)

Reconstitution:
Reconstitute with sterile dH2O to a concentration of 0.1-1mg/ml. Solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use.

Biological Activity:
Determined by its ability to induce osteoclast formation in RAW264.7 cells using a concentration range of 5.0-10.0 ng/ml
The ED50 as determined by the dose-dependent stimulation of IL-8 production by human PBMC (peripheral blood mononuclear cells) is 10ng/ml.

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
20kD

Source: Human, recombinant expressed in E. coli
Purity: 98% by SDS-PAGE and HPLC analysis.
Form: Supplied as a lyophilized powder in PBS, pH 7.6.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.