Technical Data
R1075-02
RANK (Receptor Activator of NF-kB)
Description:
Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. Receptor activator of NF-kB (RANK) is a recently cloned member of the TNFR superfamily with no significant homology to other members of this family (1). RANK ligand (RANKL/TRANCE/ OPGL) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival (2). The cytoplasmic domain of RANK interacts with TRAF2, TRAF5 and TRAF6 (3,4). Overexpression of RANK activates NF-kB and c-Jun-terminal kinase (JNK) pathways (3). Recent studies have shown that RANK interaction with TRAF6 activates NF-kB, whereas JNK activation is mediated through binding of RANK to TRAF2 (4).

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG14H238Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Fusion protein containing amino acid residues 326-616 of human RANK
Purity:
Purified
Form
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity:
Recognizes an epitope located between amino acid residues 330-427 of human RANK.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Anderson, D.M, et al., Nature 390: 175-179 (1997). 2. Wong, B.R., et al., J. Exp. Med. 186: 2075-2080. 3. Darnay, B.G et al., J. Biol. Chem 273: 20551-20555 (1998). 4. Darnay, B.G. et al., J. Biol. Chem . 274: 7724-7731 (1999). 5. Fiumara, P., et al., Younes, A. Blood , 98: 2784-2790 (2001).
6. Bharti AC, et al., J. Biol. Chem., Vol. 279, Issue 7, 6065-6076 (2004). (Flow application)