Technical Data
R1075-07
RANK (Receptor Activator of NF-kB) (PE)
Description:
Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. Receptor activator of NF-kB (RANK) is a recently cloned member of the TNFR superfamily with no significant homology to other members of this family (1). RANK ligand (RANKL/TRANCE/ OPGL) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival (2). The cytoplasmic domain of RANK interacts with TRAF2, TRAF5 and TRAF6 (3,4). Overexpression of RANK activates NF-kB and c-Jun-terminal kinase (JNK) pathways (3). Recent studies have shown that RANK interaction with TRAF6 activates NF-kB, whereas JNK activation is mediated through binding of RANK to TRAF2 (4).

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 0.25-1ug/1x10e6 cells
The epitoipe range recognized by R1075-07 is a potential cytoplasmic region as defined by uniprot.org. Refer to Bharti et al. publication and the bioinformatics data base for additional information in order to determine the suitablility for cell surface flow cytometry.
Optimal dilutions to be determined by the researcher.

Recommended Positive Control:
RAW mouse cells, fixed and permeabilized with acetone, can be used as positive control.

Storage and Stability:
May be stored at 4C before opening. DO NOT FREEZE! Stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4C. PE conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG1,k4H238Affinity Purified
SizeStorageShippingSourceHost
100ug4C Do Not FreezeBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Fusion protein containing amino acid residues 326-616 of human RANK (cytoplasmic domain)
Purity:
Purified by Protein G Affinity Chromatography.
Form
Supplied as a liquid in PBS, 0.05% sodium azide. Labeled with R-Phycoerythrin (PE).
Specificity:
Recognizes an epitope located in the cytoplasmic domain between amino acid residues 330-427 of human RANK. Species crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Anderson, D.M, et al., (1997) Nature 390:175-179. 2. Wong, B.R., et al. J. Exp. Med. 186:2075-2080. 3. Darnay, B.G., et al., (1998) J. Biol. Chem 273:20551-20555. 4. Darnay, B.G., et al., (1999) J. Biol. Chem. 274:7724-7731. 5. Evidence That Receptor Activator of Nuclear Factor (NF)-B Ligand Can Suppress Cell Proliferation and Induce Apoptosis through Activation of a NF-B-independent and TRAF6-dependent Mechanism. Alok C. Bharti, Yet al., J. Biol. Chem., Feb 2004; 279: 6065 - 6076. 6. Functional expression of receptor activator of nuclear factor B in Hodgkin disease cell lines. Fiumara P, et al., Blood, 98: 2784-2790 (2001