Technical Data
R1075-10P
RANKL (Receptor Activator of Nuclear Factor kappa B Ligand, Receptor Activator of NFkB Ligand, CD254, hRANKL2, hRANKL-2, Osteoclast Differentiation Factor, ODF, Osteoprotegerin Ligand, OPGL, sOdf, SOFA, TNF-related Activation-induced Cytokine, TRANCE, Tum
Description:
RANKL is a recently identified member of tumor necrosis factor family. TRANCE (TNF-related activation-induced cytokine receptor) or RANKL {receptor activator of NF-kB (RANK) ligand} has been implicated in interactions between T cells and dendritic cells. RANK ligand binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival. RANKL is also important in T- and B-cell maturation. As OPGL/ODF (Osteoprotegerin Ligand/Osteoclast differentiation factor), the same protein can both activate mature osteoclasts and mediate osteoclastogenesis. OPGL/TRANCE deficient mice show severe osteoporesis and complete absence of osteoclasts as a result of lack of osteogenesis.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C before opening. DO NOT FREEZE! Stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 6 months at 4C.
TypeIsotypeCloneGrade
MabIgG1,k11E334Affinity Purified
SizeStorageShippingSourceHost
100ug4C Do Not FreezeBlue IceMouseMouse
Concentration:
~0.5mg/ml
Immunogen:
Fusion protein corresponding to aa1-317 of mouse TRANCE, bacterially expressed.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide. Labeled with Biotin.
Specificity:
Recognizes mouse RANKL Species Crossreactivity: human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Anderson, D.M, et al. Nature 390: 175-179 (1997). 2. Wong, B.R., et al. J. Exp. Med. 186: 2075-2080.
3. Kong, Y-Y., et al. Nature 397: 315-323 (1999). 4. Lacey, D.L., et al. Cell 93: 165-176 (1998).