Technical Data
sRANKL, Recombinant, Human (soluble RANK Ligand, Receptor Activator of NFkB Ligand)
Molecular Biology Storage: -20°CShipping: Blue Ice
Recombinant corresponding to human sRANKL expressed in E. coli.

Molecular Weight:
~20kD (corresponding to the full length of the TNF-like extracellular domain)

Biological Activity:
ED50 <10ng/ml. The biological activity was determined by measuring the dose-dependent stimulation of IL-8 production by isolated human PBMC’s. sRANKL, also known as ODF, TRANCE and TNF superfamily member 11, is a member of the TNF ligand superfamily. The receptor for sRANKL is RANK (Receptor Activator of NF-KappaB), a member of the TNFR family identified as a dendritic cell membrane protein. sRANKL influences dendritic cells’ ability to stimulate T-cells.

Endotoxin: ~0.1ng/ug

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Purity: ~98% (SDS-PAGE, HPLC)
Concentration: Not determined
Form: Supplied as a lyophilized powder in 5mM Tris, pH 7.6, and 50mM sodium chloride. Reconstitute with sterile dH2O to 0.1-1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.