Technical Data
RANTES, Recombinant, Rat (Regulated on Activation, Normal T-cell Expressed and Secreted)
Molecular Biology Storage: -20CShipping: RT
RANTES is a protein which has been shown to be a chemo-attractant for peripheral blood monocytes. It appears to selectively attract T cells of the CD4+/CD45RO+ phenotype in vitro. Rat RANTES is a 7.9kD protein containing 68 amino acid residues.

Biological Activity:
Rat Rantes produces chemotaxis over a wide concentration range in assay using rat peritoneal macrophages. Significant chemotaxis was achieved using a concentration of 50.0-100ng/ml.

Responding Cells (partial list): Monocytes, T cells, others

Amino Acid Sequence:

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: E. coli
Purity: 98% (SDS-PAGE and HPLC analysis). Endotoxin: 0.1ng/ug
Form: Supplied as a lyophilized powder with no additives. Reconstitute with sterile ddH2O to a concentration of 0.1-1.0mg/ml. Do not vortex. For long term add protein carrier. It is recommended that further dilutions be made into other aqueous buffers.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.