Technical Data
R1120-60
RAP2A (RAP2A Member of RAS Oncogene Family, Ras-related Protein Rap-2a, RAP2, KREV, K-REV, RbBP-30)
Description:
RAP2A is a signaling protein involved in small GTPase mediated signal transduction activated by guanine nucleotide-exchange factors (GEF) EPAC and EPAC2, in a cAMP-dependent manner, and GFR. This membrane protein belongs to the small GTPase superfamily and Ras family and contains domains required for GTP binding and a Ras-like C-terminal motif for post-translational lipid binding and subsequent anchoring to the plasma membrane. Apart from having a possible role in membrane shedding, RAP2A also functions as a slowly responding molecular switch in the RAP1A signaling cascade and also interacts with ARHGAP29, the putative specific effector of RAP2A.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1-0.3ug/ml
Immunohistochemistry (formalin fixed paraffin embedded): 5ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
MCF7 cell lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa140-190 of human RAP2A.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity:
Recognizes human RAP2A. Species Crossreactivity: chicken, canine, equine, money, mouse, opossum, xenopus. Species sequence homology: chimpanzee, pufferfish, zebrafish.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Lerosey, I. et al. J. Biol. Chem. 266:4315-4321 (1991). 2. Pizon, V. et al. Oncogene. 3:201-204 (1988). 3. Rousseau-Merck, MF. et al. Cytogenet. Cell Genet. 53:2-4 (1990). 4. Zhu, Y. et al. Neuron. 46:905-916 (2005). Note: Erratum: Neuron. 47:321 only, 2005.