		Technical Data
R1120-60	RAP2A (RAP2A Member of RAS Oncogene Family, Ras-related Protein Rap-2a, RAP2, KREV, K-REV, RbBP-30)

Description:
RAP2A is a signaling protein involved in small GTPase mediated signal transduction activated by guanine nucleotide-exchange factors (GEF) EPAC and EPAC2, in a cAMP-dependent manner, and GFR. This membrane protein belongs to the small GTPase superfamily and Ras family and contains domains required for GTP binding and a Ras-like C-terminal motif for post-translational lipid binding and subsequent anchoring to the plasma membrane. Apart from having a possible role in membrane shedding, RAP2A also functions as a slowly responding molecular switch in the RAP1A signaling cascade and also interacts with ARHGAP29, the putative specific effector of RAP2A.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1-0.3ug/ml
Immunohistochemistry (formalin fixed paraffin embedded): 5ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
MCF7 cell lysate

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide corresponding to aa140-190 of human RAP2A.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

Specificity:

Recognizes human RAP2A. Species Crossreactivity: chicken, canine, equine, money, mouse, opossum, xenopus. Species sequence homology: chimpanzee, pufferfish, zebrafish.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Lerosey, I. et al. J. Biol. Chem. 266:4315-4321 (1991). 2. Pizon, V. et al. Oncogene. 3:201-204 (1988). 3. Rousseau-Merck, MF. et al. Cytogenet. Cell Genet. 53:2-4 (1990). 4. Zhu, Y. et al. Neuron. 46:905-916 (2005). Note: Erratum: Neuron. 47:321 only, 2005.