Technical Data
Red Blood Cells (RBC)
The high-affinity anti-RBC monoclonal antibody efficiently bind to Fc receptors on macrophages inducing anemia in vivo due to a rapid Fc receptor (FcyR)-mediated erythrophagocytosis of opsonised RBC in spleen and livers. The capacity of the antibody to interact with FcyR is responsible for its haemolytic activity.

Suitable for use in Flow Cytometry and Functional Studies. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:50
Optimal dilutions to be determined by the researcher.

Positive Control: Mouse erythrocytes

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG2a13A66Affinity Purified
100ug-20CBlue IceMouseMouse
Mouse red blood cells
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, 0.1% BSA.
Recognizes mouse RBC. Does not react with rat, sheep, rabit, chicken and human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Shibata, T et al; Monoclonal anti-erythrocyte autoantibodies derived from NZB mice cause autoimmune haemolytic anemia by two distinct pathogenic mechanisms. Int. Immun. 1990, 2:1133. 2. Fossati-Jimack, L et al; High pathogenic potential of low-affinity autoantibodies in experimental autoimmune haemolytic anemia. J. Exp. Med. 1990, 190:1689. 3. Fossati-Jimack, L et al; Selective increase of autoimmune epitope expression on aged erythrocytes in mice: Implications in anti-erythrocyte autoimmune responses. J. Autoimmun. 2002, 18:17. 4. Azeredo da Silveira, S et al; Complement activation selectively potentiates the pathogenicity of the IgG2b and IgG3 isotypes of a high-affinity anti-erythrocyte autoantibody. J. Exp. Med. 2002, 195:665. 5. Baudino, L et al; Differential contribution of three different activating IgG Fc receptors (FcyRI, FcyRIII and FcyRIV) to IgG2a- and IgG2b-induced autoimmune haemolytic anemia in mice. J. Immunol. 2008, 180:1948.