Description:
The high-affinity anti-RBC monoclonal antibody efficiently bind to Fc receptors on macrophages inducing anemia in vivo due to a rapid Fc receptor (FcyR)-mediated erythrophagocytosis of opsonised RBC in spleen and livers. The capacity of the antibody to interact with FcyR is responsible for its haemolytic activity.
Applications:
Suitable for use in Flow Cytometry and Functional Studies. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 1:50
Optimal dilutions to be determined by the researcher.
Positive Control: Mouse erythrocytes
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG2a | 13A66 | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 100ug | | -20°C | Blue Ice | Mouse | Mouse |
|
| Concentration: |
| ~0.1mg/ml |
| Immunogen: |
| Mouse red blood cells |
| Purity: |
| Purified by Protein G affinity chromatography. |
| Form |
| Supplied as a liquid in PBS, 0.1% BSA. |
| Specificity: |
| Recognizes mouse RBC. Does not react with rat, sheep, rabit, chicken and human. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
1. Shibata, T et al; Monoclonal anti-erythrocyte autoantibodies derived from NZB mice cause autoimmune haemolytic anemia by two distinct pathogenic mechanisms. Int. Immun. 1990, 2:1133. 2. Fossati-Jimack, L et al; High pathogenic potential of low-affinity autoantibodies in experimental autoimmune haemolytic anemia. J. Exp. Med. 1990, 190:1689. 3. Fossati-Jimack, L et al; Selective increase of autoimmune epitope expression on aged erythrocytes in mice: Implications in anti-erythrocyte autoimmune responses. J. Autoimmun. 2002, 18:17. 4. Azeredo da Silveira, S et al; Complement activation selectively potentiates the pathogenicity of the IgG2b and IgG3 isotypes of a high-affinity anti-erythrocyte autoantibody. J. Exp. Med. 2002, 195:665. 5. Baudino, L et al; Differential contribution of three different activating IgG Fc receptors (FcyRI, FcyRIII and FcyRIV) to IgG2a- and IgG2b-induced autoimmune haemolytic anemia in mice. J. Immunol. 2008, 180:1948.
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