Technical Data
R1310-01A
Red Fluorescent Protein (RFP)
Description:
Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the peptide epitope tag. The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the peptide tag. Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered peptide, thus eliminating the need for antibodies to proteins from each newly cloned gene.

Applications:
Suitable for use in ELISA, Western Blot, Dot Blot, Immunoprecipitation and Immunostaining. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG110K338Ascites
SizeStorageShippingSourceHost
100ug-20CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
Red Fluorescent Protein (RFP) from Discosoma and anemone N-terminal peptide, conjugated to KLH.
Purity:
Purified
Form
Supplied as a liquid in 10mM PBS, pH 7.2, 0.1% sodium azide.
Specificity:
Recognizes native and denatured forms of RFP and its variants tag-RFP, turbo-RFP, DeRed, mCherry and mOrange.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Prasher D.C. et al., Gene 111: 229-233 (1992). 2. Chalfie M. et al., Science 263: 802-805 (1994)