Technical Data
Red Fluorescent Protein (RFP)
Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the peptide epitope tag. The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the peptide tag. Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered peptide, thus eliminating the need for antibodies to proteins from each newly cloned gene.

Suitable for use in ELISA, Western Blot, Dot Blot, Immunoprecipitation and Immunostaining. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ug-20CBlue IceMouse
Red Fluorescent Protein (RFP) from Discosoma and anemone N-terminal peptide, conjugated to KLH.
Supplied as a liquid in 10mM PBS, pH 7.2, 0.09% sodium azide.
Recognizes native and denatured forms of RFP and its variants tag-RFP, turbo-RFP, DeRed, mCherry and mOrange.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Prasher D.C. et al., Gene 111: 229-233 (1992). 2. Chalfie M. et al., Science 263: 802-805 (1994)